BLACK LEG 249 
homogeneous mass. This is spread in a thin layer on a suitable glass 
dish and placed in an oven, the temperature of which may be regu- 
lated with exactness. The reason for mixing the dried muscle with 
water is to insure a quicker and more uniform attenuation. The 
temperature of the oven is previously brought up to 95° to 99° C., and 
the virus is allowed to remain in it for six hours. When removed it 
appears as a brownish scale, which is easily detached from the dish. 
This scale is pulverized and put up in packages containing 10 doses 
each. Before it is used, it is mixed with 10 cc. water, filtered and the 
filtrate injected in doses of one cubic centimeter.* 
Specific biologic treatment. Arloing immunized a heifer by inocula- 
ting it with increasing doses of the virus over a period of six months. 
Its serum possessed prophylactic, therapeutic and antitoxic proper- 
ties. In doses of 10 cc., it protected sheep against a fatal dose of the 
virus. Leclainche and Vallée report an antiserum from hyper- 
immunized goats and horses. 
Roux, Diinschmann and Leclainche and Vallée have all demon- 
strated that a toxin is produced in cultures of B. Chauvaei. A satis- 
factory specific treatment, however, does not seem to have been 
produced. 
REFERENCES 
1. Fiscner anp Kinstzy. Blackleg in Kansas, and protective inoculation. 
Bulletin No. 105, Kan. State Agric. Exper. Station, 1901. 
2. Hooren. Rauschbrand Diagnose. Tijdsch. Veearts., Bd. XL. (Ref. Ellen- 
berger's Jahresbericht, 1914.8. 32.) 
3. Karona. Beitrage zur pathologischen Anatomie des Rauschbrand. (Ref. 
Ellenbergers Jahresbericht, 1911, 8. 39.) 
4. Lewis. Symptomatic anthrax. Bulletin No. 27. Oklahoma State Agric. 
Exper. Station, 1897. 
5. Mayo. Blackleg. Bulletin No. 69. Kan. State Agric. Exper. Station, 1897, 
p- 108. 
6. N6reaarp. Blackleg in the United States and the distribution of vaccine by 
the Bureau of Animal Industry. Annual Report of the Bureau of Animal Industry, 
U.S. Dept. Agric., 1898. 
7. NoOreaarp. Blackleg. Its nature, cause and prevention. Circular No. 23. 
U. S. Bureau of Animal Industry, 1898. 
8. Paquin. Blackleg. Bulletin No. 12. Mo. Agric. Exper. Station, 1890. 
9. Psrrers. Blackleg. Its nature, cause and prevention. Bulletin No. 65. 
Neb. State Agric. Exper. Station, 1900. 
.10. Satmon. Black quarter. Annual Report Bureau of Animal Industry, U. 8. 
Dept. of Agric., 1893-4. 
*Leclainche and Vallée have described a method of making a vaccine from pure 
cultures. 
