: ACTINOBACILLOSIS Q71 
preparation. It stains with the ordinary aniline dyes.* It does not 
take the Gram stain. 
In the fresh tissues or in sections it appears in granules the same as 
in actinomycosis. 
The actinobacillose is virulent for guinea pigs and rabbits. When 
inoculated into the abdominal cavity with pure cultures guinea pigs 
die in from nineteen to thirty-one days (Higgins) of generalized 
actinobacillosis. These are reported to be characteristic and different 
from those of any of the other observed infective agents. According 
Fic. 63. A PHOTOGRAPH OF A SECTION OF A TUMOR STAINED BY GRAM’S METHOD BUT 
NOT FULLY DECOLORIZED. xX ABOUT 1,000. (AFTER HIGGINS). 
to Higgins, “Small pearly-white nodules appear just beneath the peri- 
toneal and pleural membranes, varying from 1.0 to 5.0 mm. in diame- 
*Higgins recommends the Romanowsky stain as modified by Dutton and Todd 
for sections or preparations from pus. The formula for the stain and method for its 
use are as follows: 
Stain: Eosin, aqueous solution.............. 0.0 cece cece e eee ee 1 part 
ROPE ELS TBC aysiasinc vcd set rsd tice adits de ee A toed vaustuesbniaon ay eae toast ot co 
Watt acc samdaentatiaciw mapas aan ements au cae 8 parts 
Mix just before using and filter. Suspend the preparations (sections fixed to the 
slide) upside down on the stain to saturate them from below, to avoid percipitate. 
Stain in this solution for thirty minutes. Wash thoroughly in water, then in a 10% 
solution of tannic acid, which will brighten the color, and again wash in water. Dehy- 
drate in alcohol, clear and mount on xylol balsam. The stain as above prepared 
spoils quickly. 
