290 EPIZOOTIC LYMPHANGITIS 
Valerio consider it as belonging to the protozoa, and Formi and Aruch 
as a blastomycete. Tokishige and Marconi believe that it belongs 
with the saccharomyces. It is not easily stained by the aniline dyes, 
although Mettam has shown that by the Gram method, Nicolle’s 
violet, Nicolle’s thionine and others it is readily colored. It is culti- 
vated with difficulty. Tokishige obtained cultures in bouillon, agar, 
gelatin and on potato. In bouillon it required seventeen days to 
obtain a growth. 
The infectious material may be transported by contact between the 
diseased and well horses, by stall bedding, by stable utensils and 
harnesses and possibly by insects. 
The period of incubation is placed at from three weeks to three 
months and in certain cases it may extend to even eight or ten 
months. Inexperimental cases symptoms have appeared after 32 days. 
Symptoms. An infection takes place in wounds, the first symptom 
usually appearing at the seat of a pre-existing wound. The lesions 
usually appear in the skin, but they may occur on a mucous mem- 
brane. They consist of swelling and suppuration of the lymph 
vessels and glands. These break and discharge a thick, yellow pus, 
stained with blood. Pearson states that the horses do not, as a rule, 
show any general disturbance except in very advanced cases. Pallin 
describes the opened sores as follows: 
“The buds, ulcers, or sores, by all of which names they are known, 
are characterized by their bright red exuberant granulations and their 
fungoid appearance, as well as by their indurated base and well- 
defined edges; the adjoining skin, which is partially inverted, has 
a peculiar shiny appearance; an opening exists in the center of the 
bud, from which the pus, at first creamy, and afterwards yellowish, 
oily, and curdled, is continually discharging.” 
These buds are quite different from those of glanders. The 
lesions are commonest in the limbs. The most usual location is on 
the fore-leg generally extending up along the fore-arm to the brachial 
region and point of the shoulder. 
The sores vary in size from that of a pea to a hen’s egg. Pallin 
reports lesions on the mucous membranes in from 7 to 10 per cent 
of the cases. When these occur on the nasal mucosae they are liable 
to be confounded with those of glanders. 
Usually the general symptoms are not conspicuous. The tempera- 
ture remains normal and the appetite good. The disease seems to 
thrive best on animals in good condition. 
