CANINE MALARIA 545 
The histological examination of the organs when hardened in 
Miller’s fluid shows, according to Graham-Smith, the capillaries of 
the alveoli of the lungs to be dilated and in some instances a prolifera- 
tion of the lining cells some of which are seen lying free in the alveoli. 
In other alveoli the process is more advanced and proliferated cells, 
leucocytes, and in some cases red corpuscles are present in them. The 
lumen of many of the bronchioles frequently contains desquamated 
epithelium, leucocytes and mucus. There is no evidence of any 
increase in the connective tissue. No pigmentary or fatty changes 
were observed in the heart or skeletal muscles. The liver showed the 
most marked changes. The central vein of the lobule and the capil- 
laries lying between the liver cells were dilated. The protoplasm of 
the liver cells stains feebly but the nuclei take the stain fairly well. 
The cells are distorted between the dilated blood vessels and in many 
cases almost destroyed, especially those in the central zone. The 
vessels in the interlobular spaces are dilated but the bile ducts are 
normal. There is no increase of fibrous tissue and the capsule is 
normal. The capillaries contain a large number of red blood cor- 
puscles and the proportion of leucocytes is high. The latter are also 
very numerous in the larger vessels. In these vessels about 10% of 
the red corpuscles are infected. The parasites usually appear in 
small groups in the cells. The proportion of leucocytes to red 
corpuscles is about 1 to 10. In the capillaries from 23 to 53% of the 
red corpuscles are infected and in some instances the proportion of 
leucocytes to red corpuscles is as high as 1 to 3. In but one dog did 
he find fatty changes. The smear preparations showed that 98.9% 
of all infected corpuscles contained one to four parasites and the 
balance more than four parasites. Free parasites were found in the 
proportion of 1 to 214 infected corpuscles. The spleen pulp contains in 
most cases a large quantity of blood and the vessels in the trabecule 
are dilated and contain numerous leucocytes. The proportion of 
infected corpuscles in the pulp is small (3 to 12%) but in the small 
trabecular veins it is high (48%). In the kidney there were no changes 
that were constantly present except dilatation of the blood vessels. 
In some cases about 46% of the red corpuscles in the vessels of the 
glomeruli were infected. 
Diagnosis. The positive diagnosis is made by finding the parasite 
in the red blood corpuscles. The symptoms are not very conclusive. 
Piroplasmosis is to be differentiated from distemper and from an 
anemia and disintegration of red corpuscles, hyperleucocytosis, 
