TRYPANOSOMA 357 
observe conjugation. The chromatin divides into from 3 to 10 seg- 
ments, which assume irregular shapes and locations, some of which are 
often found well up in the flagellum. The nucleus usually divides 
into equal parts, but may break into several segments. After the 
nuclear division the protoplasm may assume various irregular forms. 
The young nuclei arrange themselves in groups, and the parasite 
twists and splits by longitudinal or more often by transverse 
fission. The new forms resulting from the division soon assume 
7 8 9 10 1 
Fig. 85. 2, TRYPANOSOMA LEWISI COMPLETELY DEVELOPED; n, NUCLEUS: Cc, CENTRO- 
SOME; ™, UNDULATING MEMBRANE; f, FLAGELLUM. 3-6, TRYPANOSOMA IN PROCESS 
OF DIVISION. 7-10, OTHER FORMS OF MULTIPLICATION OF TR. LEWISI, 11, FLAGELLA 
NOT STAINED. XX aBoutT 2,000 pramETeERs (after Laveran and Mesnil). 
the regular shape. Plimmer and Bradford consider longitudinal and 
transverse division the more frequent modes of reproduction. They 
observed conjugation, which consisted in the fusion of the micronuclei, 
followed by an amceboid stage and division by segmentation. The 
order of division appears to be (1) centrosome, (2) flagellum, and (3) 
nucleus and protoplasm. Other forms of reproduction have been 
described by Martini, Laveran and Mesnil and others. Involution 
