DOURINE 365 
This coagulates the albumen and makes a permanent film in which the corpuscles and 
organisms are retained. Remove from the alcohol, wash in water, and then apply the 
stain. This is made by mixing equal parts of the above two solutions, freshly prepared, 
in a small glass measure or porcelain dish. It is important that the admixture should 
be as fresh as possible. Apply the stain to the whole of the film and let it remain for 
seven to ten minutes; wash in water and dry in the air, no heat being applied. The red 
corpuscles may have a bluish tinge, which may be removed by further washing. If 
the blood platelets appear bluish the film requires further staining; they should appear 
as ruby-red granular bodies. By continuous application of water the stain may be 
washed out, but the film may always be stained over again. 
By this stain the protoplasm of the trypanosome is stained blue, the nuclear chroma- 
tin a carmine violet, and the flagellum and centrosome a brilliant red. The red 
corpuscles will be a pinkish color, and the various forms of leucocytes will be well 
differentiated. In examining the smear, time may be saved by looking along the edge 
of the film, as it is here that the parasites will be most numerous if they are present, as 
they, being like the leucocytes of less density than the rest of the blood, tend to run to 
the periphery when the smear is made. 
A film made in this way requires no cover-glass, but if the cedar oil is left on it tends 
to withdraw the color; however, if it be carefully blotted and wiped off with a soft rag 
after use the film may be kept indefinitely. 
The period of incubation in naturally infected animals is placed by 
different authors at from 2 to 60 days. Cases are reported, however, 
where symptoms did not appear for 12 months. 
Symptoms. The first symptom in the stallion consists in the 
swelling of the glans penis. Reddish spots, vesicles and ulcers on the 
outer surface of the organ. The meatus urinarius is reddened and 
swollen with a mucous discharge. Later small yellowish-red nodules 
develop singly or in groups on the mucosa at the opening of the 
urethra or on the glans itself, which may ulcerate and, healing, leave 
whitish areas. The animal has a continuous desire to micturate and 
frequently manifests sexual excitement. The swelling also spreads 
from the penis to the sheath and scrotum in which case the testicles 
become inflamed. Finally, the inguinal glands and lymph vessels 
become involved. These local affections may, as the disease advances, 
almost entirely disappear. In some cases the external changes are 
absent, as the mucous membrane of the urethra is first affected, the 
only visible symptoms being strangury and a mucous discharge from 
the urethra. 
In mares, the disease begins with a doughy or tense swelling of the 
pudendum, which frequently spreads to the udder and inner surface 
of the thighs. The vulva may be sprinkled with non-pigmented 
spots. The mucous membrane of the vagina is red in spots and 
