INFECTIOUS DISEASES OF CATTLE. 417 



3. That tuberculin has no injurious effect upon healthy cattle. 



4. That the comparatively small number of cattle which have 

 aborted, suffered in health, or fallen off in condition after the tuber- 

 culin test were either diseased before the test was made or were 

 affected by some cause other than the tuberculin. 



SUMMARY Off DIRECTIONS FOR MAKING THE TUBERCULIN TEST. 



1. Stable cattle under usual conditions and among usual surround- 

 ings, feed and water in the customary manner. 



2. Make a physical examination of each animal, and give to each 

 one some designation by which the animal will be known throughout 

 the test. 



3. Take each animal's temperature at least three times at two or 

 three hour intervals on the day of injection; for instance, at 2, 5, 

 and 8 p. m. 



4. At 8 or 10 p. m. inject a dose of tuberculin under the skin in the 

 region of the shoulder, using a sterile hypodermic syringe after dis- 

 infecting the skin at the seat of injection with a 5 per cent solution of 

 carbolic acid or a similar antiseptic solution. 



5. Tuberculin is not always concentrated to the same degree and 

 therefore the dose, which should always appear on the label, varies 

 considerably. The dose of imported tuberculin is 0.25 c. c. for an 

 adult cow, and before injection is diluted with sterile water to 2 c. c. 

 The tuberculin made by the Bureau of Animal Industry is prepared 

 so that it will not be necessary to dilute it, and the dose is 2 c. c. for 

 an adult animal. Yearlings and 2-year-olds, according to size, should 

 receive from 1 to 1-J c. c, while bulls and very large animals may 

 receive 3 c. c. 



6. The next day, at 6 a.m., commence taking temperatures, and con- 

 tinue every two or three hours until the twentieth hour after injection, 

 at which time if there is no tendency for the temperature to rise the 

 test may cease. 



7. A rise of two or more degrees Fahrenheit above the maximum 

 temperature observed on the previous day, providing this temperature 

 exceeds 103.8° F., should be regarded as an indication of tuberculosis. 

 Those cases which approximate but do not reach this standard should 

 be considered as suspicious and held for a retest six weeks later, 

 giving double the original dose. 



TREATMENT OF TUBERCULOSIS. 



Treatment of the disease is not seriously considered by any authori- 

 ties at the present time. 

 The measures to be adopted to prevent the spreading of the disease 



61386— OS 27 



