INFECTIOUS DISEASES OF CATTLE. 425 



tested of tuberculosis in children would by Koch's method be classified 

 as of bovine origin. 



In the Bureau of Animal Industry two distinct lines of experiments 

 have been carried on, in order that one might serve as a check against 

 the other. There has been, however, no discrepancy in the results. 

 De Schweinitz, in the Biochemic Division, Bureau of Animal Indus- 

 try, has isolated 9 cultures from human tuberculosis. Two of these 

 were derived from human sputum, 3 from cases of generalized tuber- 

 culosis in adults, and 4 from cases of generalized tuberculosis in chil- 

 dren. By comparing these cultures with a newly isolated virulent 

 culture of bovine tuberculosis, there were found among them 2 cultures 

 from children which were identical in their cultural and morphological 

 characters with the bovine bacillus. These cultures also killed rabbits 

 and guinea pigs in as short a time as did the bovine bacillus. Hogs 

 which were inoculated subcutaneously with these 2 cultures from chil- 

 dren died of generalized tuberculosis. Two calves weighing over 300 

 pounds each were inoculated subcutaneously with these virulent human 

 cultures, and as a result developed generalized tuberculosis. A year- 

 ling heifer inoculated with 1 of the cultures showed generalized tuber- 

 culosis when killed three months after inoculation. Both the cattle 

 and the hogs had been tested with tuberculin and found to be free 

 from tuberculosis before the inoculations were made. It is important 

 to observe in this connection that 2 out of 4, or 50 per cent, of the 

 cultures obtained from cases of generalized tuberculosis in children 

 proved virulent for cattle. 



Mohler, working in the Pathological Division, Bureau of Animal 

 Indue- try, has obtained 3 very virulent cultures of tubercle bacilli 

 from the human subject. A goat inoculated subcutaneously with 1 

 of these cultures died in thirtj'-seven days with miliary tuberculosis of 

 the lungs involving the axillary and prescapular glands. This bacillus 

 was obtained from the mesenteric gland of a boy. Of still greater 

 interest is a bacillus isolated by Mohler from human sputum. A goat 

 inoculated subcutaneously with a culture of this germ died in ninety- 

 five days of pulmonary tuberculosis. A cat inoculated in the same 

 manner died in twenty-three days of generalized tuberculosis. A 

 rabbit similarly inoculated died in fifty-nine days of pulmonary 

 tuberculosis. Another rabbit inoculated with a bovine culture for 

 comparison lived ten days longer than the one inoculated with this 

 sputum germ. Mohler also inoculated subcutaneously a 1-year-old 

 heifer with a culture derived from the tubercular mesenteric gland of 

 a boy 4 years of age. This culture was always refractory in its growth 

 under artificial conditions, and the bacilli were short, stubby rods, 

 corresponding in appearance with the bovine type. At the autopsy, 

 held one hundred and twenty-seven days after the inoculation, the 

 general condition was seen to be poor and unthrifty, and large, hard 

 tumors were found at the points of inoculation. On the right side 



