8o 



SOLID CULTIVATION MEDIA. 



wire is now withdrawn; and the cotton wool plug is firmly replaced in 

 the mouth of the test-tube. All these manipulations must be carried 



Fig 34. — Method of inoculation in. test-tubes containing nutrient 

 jelly. 



a. Cultivation in jelly, from which inoculation is to be made. 



b. Tube containing sterile jelly, into which the straight needle is 



introduced. 



c. Cotton wadding plugs held between the tips of the fingers. 

 ' (The hand is held in such a position that these plugs have 



their smooth surfaces downwards.) 



out in a perfectly still atmosphere, or under the protection of the 

 carbolic spray. The latter method specially offers many advantages : 



