PREFACE. 
The following pages constitute, substantially, the material which has been furnished the stu- 
dents in Bacteriology at the University of Wisconsin, in mimeograph form, for several years. They 
contain directions for the performance of certain fundamental exercises in Bacteriology. 
Ina rapidly developing subject it is important that the various exercises be worded so as to lend 
themselves readily to changes which become desirable from time to time. With this end in view the 
exercises have been divided, where possible, into a general and a special part. The general directions 
contain the essential part of the exercise which does not permit of any considerable variation, while 
the special directions embrace such features as are most subject to modification, as for instance, 
the particular organism to be used, the kind of medium, the incubation temperature, etc. Desirable 
changes here are easily indicated when the exercise is assigned. 
Some of the exercises can be performed in a few minutes, while others require several days 
for their completion. No attempt has been made to group them according to their length, nor to 
divide the text into lessons, but as far as possible they are arranged in the order in which they would 
be logically used in the laboratory. 
The right hand pages have been left for notes and drawings with the idea that notes in perma- 
nent form are the only ones of value to the student in subsequent years. 
The charts of the various organisms furnish a most satisfactory means for recording the observa- 
tions made during the study of a germ and are especially convenient for reference. 
Part I. is the work required of students taking the General Course in which special emphasis is 
placed on the biology of bacteria. It is completed in the first semester. Part II. which is given 
during the second semester includes the more specialized phases of the work, particularly as applied 
to the student preparing for medicine. 
References have been made to all of the leading English text-books and occasionally to original 
sources. It is expected that the student will make constant use of these references. 
My thanks are due Prof. H. L. Russell under whose general direction the work outlined here is 
given, for valuable help in the selection and arrangement of the material and for generous council, I 
am also indebted to Mr. E. G. Hastings, Assistant Bacteriologist to the Wisconsin Experiment Station, 
for critical reading of manuscript and proof. . 
WILLIAM DODGE FROST. 
MapIson, WiIs., January, 1901. 
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