CONTENTS. 
List of References...........-. 83 Sit cecusded 8 8-0 E SA MENOWE RE 400 Oi Swi eae e he spree A fds aeaiaon dash aupe Ree ise vi 
List of Apparatus. ........0. cece cece eee ee eet e ne terete erent eters f nee nee tense tence enenes vii 
Laboratory Rules........ cc eee cece ene ee een eee eee ene teen esaaea cert sree ttesanees . Vili 
PART I.—GENERAL BACTERIOLOGY. 
Cuarter I. MoreHoLtoGy AND ELEMENTARY TECHNIQUE. 
EXERCISE. PAGE. | EXERCISE. _ PAGE. 
I. Cleaning Glassware.............. 2 XX. Test-tube Cultures Illustrating 
II. Plugging Flasks and Tubes....... 2 . Form Types s4 2 ssieseuws s+ oss 26 
III. Sterilization of Glassware........ 2 XXI. Study of Test-tube Cultures 26 
IV. Preparation of Bouillon ......... 4 XXII. Microscopical Study of - Forn 
V. Filling Test-tubes and Flasks with TY POS ec asssecieeny cetera! wos eee ace 26 
Culture Media ................ 6 XXIII. Drawing Bacteria...............- 28 
VI. Sterilization of Culture Media.... 8 XXIV. Study of Cell Grouping .......... 30 
VII. Preparation of Gelatin. ........ 10 XXV. Study of Involution Forms....... 30 
VIII. Preparation of Agar............. Io XXVI. Gelatin Plate Cultures........... 32 
IX. Preparation of Potatoes. ....... 12 XXVIL. Agar Plate Cultures ............. 34 
X. Preparation of Water- blanks nigh as 12 XXVIII. Roll Cultures ......... .... 202008 34 
XI. Care of Culture Media..>........ 14 |. XXIX. Study of Plate Cultures.......... 36 
XII. Platinum Needles ............... 14 XXX. Use of Decolorizing Agents ...... 36 
XIII ' Test-tube Cultures. ...5.,........ 14 XXXKI. Gram's Stain ..........0.02 sees 36 
XIV. Incubation of Cultures. % . 16 XXXII. Tubercle Stain (Gabbett)......... 38 
XV. Cleaning Slides and Cover- glasses. 18 XXXIII. Staining Endospores............. 38 
XVI. Preparation of Staining Solutions. 18 XXXIV. Study of Endospores .... ....... 40 
XVII. Simple Cover-glass Preparation... 20 XXXV. Flagella Stain...........---0..0. 40 
XVIII. Use of Microscope .. .......... 22 XXXVI. Capsule Stain.... .............. 42 
XIX. Hanging-drop Breparehion: ocr 24 
CuarTer II. PxHystoLoGy oF BACTERIA. 
EXERCISE. PAGE. | EXERCISE, PAGE. 
XXXVIL. Preparation of Special Media..... 44 XLVII. Detection of Gas............... 50 
XXXVIII. Effect of Reaction of Media on XLVIII. Quantitative Analysis of Gas . 50 
GROWN onic cis oa Wnaewon nies 44 XLIX. Detection of Acids ............ 52 
XXXIX. Effect of Concentration of Media L. Quantitative Determination :of Acids 52 
on. Growth 2 66. ..608 senenes 46 LI. Detection of Nitrites in Cultures... 52 
XL. Effect of Temperature Variations LII. Detection of Ammonia....... .. 52 
on Rate of Growth............. 46 LILI. Detection of Sulphuretted Hy- 
XLI. Determination of Thermal Death TORBEN cris. teas ss aipails va sistas 54 
FOUR, coal veenmens bok eend omeee 46 LIV. Detection of Indol............... 54 
XLII. Comparative Efficiency of Dry and LV. Determination of Chemical En- 
Moist’Heéat 0.2 2 ss cesinee eg ee 48 zymes in Cultures ...........6. 54 
XLIIL. Effect of Desiccation ............ 48 LVI. Variation in Enzyme Production... 54 
XLIV. Effect of Chemicals on Bacteria... 48 LVII. Variation in Color Production.... 56 
XLV. Relation to Oxygen............ +. 50 : 
XLVI. Effect of Direct Sunlight ........ 50 
CuapTerR III, Taxonomy. 
PAGE, 
Points to be observed in the study of Bacteria.. 57 | Classification of Bacteria (Migula)............. mao 
CuapTeR IV. SysTEMATIc Stupy oF REPRESENTATIVE Non-PATHOGENIC BACTERIA. 
EXERCISE. ; : PAGE. | EXERCISE. PAGE 
LVIII. Preparation of Special Media..... 63 LXII. Bacterium phosphorescens ....... 68 
~ LIX. Bacillus prodigiosus ore eee 64 LXIII. Bacillus acidi lactici:........... , 70 
LX. Variety of Pigments ............. 66 LXIV. Bacillus vulgaris ...........0... 72 
LXI. Separation of Bacterial Coloring 
Mattef wa ieanas cetncamaaaa dick ce 67 
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