LABORATORY RULES. 
I. Food should not be eaten in the laboratory and lead pencils or labels should 
not be moistened with the tongue. 
II. All possible cleanliness should be observed in the care of apparatus, desk, 
ete. 
Ill. The platinum needles used in making cultures should be sterilized shortly 
before and immediately after use and before they are laid down. When the 
needles are covered with infectious material they should be held at the side of 
the flame until dry before being sterilized; this will avoid the danger of scatter- 
ing this material about the laboratory. 
IV. If infectious matter should by accident come in contact with the hands or 
be dropped on the table or floor, corrosive sublimate (1:1000) should be imme- 
diately applied. 
V. Solid material, culture media and corrosive sublimate should not be put 
in the sink but in crocks provided for the purpose. Burnt matches, pieces of 
paper, ete., should also be put in the crocks and not on the floor. 
VI. All cultures of bacteria should be labeled with the name of the organism, 
the name of the student and the date. 
VII. Discarded cultures should be covered with corrosive sublimate and placed 
in a proper receptacle and under no condition should they be left lying about 
the laboratory. Pipettes which have been used to handle infectious material 
should be placed in a glass cylinder containing a disinfectant or potassium bi- 
chromate and sulphuric acid. 
VIII. When using the steam sterilizer see that there is enough water present 
before lighting the gas and do not leave the laboratory until the gas has been 
turned off. ‘is 
IX. Before beginning an exercise read over the directions and look up some 
of the references. Keep notes of everything done and the conclusions reached 
on the right hand pages in this Guide. Make drawings wherever they will be 
of value. Outline with pencil and fill in with India ink. The laboratory Guide 
should be kept in the laboratory. 
X. At the close of the day’s work the tables should be washed with corrosive 
sublimate and the hands disinfected by washing in the sublimate solution (or a 
germicidal soap) and then in soap and water. 
[viii] 
