General Bacteriology. 59 
d. Character of whey: clear; turbid; flocculent. 
e. Reaction. 
f. Gas. 
g. Odor. 
8. Buoop Serum: (Same as streak cultures). 
MORPHOLOGICAL CHARACTERS. 
a. Form. 
b. Cell grouping. 
c. Size. 
1. In terms of the micromillimeter; breadth, average and extreme length. 
2. In terms of human blood cell. 
d. Stain. 
1. Aqueous solutions; stains easily or with difficulty; uniformly or irreg- 
ularly. ; : 
2. Speéial stain; Gram; tubercle; ete. 
e. Motility. 
1. Brownian movement. 
2. Vital movement; sluggish or active; rotary or direct; most favorable 
temperature ; age; media; ete. 
3. Flagella; stained by howtos, Bunge or Van Ermengem’s method; dis- 
tribution, monotrichal, lophotrichal or peritrichal. 
f. Capsule; stained by Ziehl, Gram or Welch’s method; most favoriile con- 
ditions; broad or narrow; present inserum, milk or on agar streaks. 
g- Spores; time required for formation; media; position in cell, center or end; 
effect on shape of cell, clostridium, or drumstick; germination, time, temperature; stain, 
Hauser or Moeller’s method; temperature limits. 
h. Vacuoles (plasmoloysis). 
1. Crystals. 
j. Involution forms. 
k. Pleomorphism. 
1. Effect of various media. 
2. Effect of reaction of media. 
PHYSIOLOGICAL CHARACTERS. 
. Effect of desiccation. 
. Relation to temperature; minimum; optimum; maximum; thermal death 
oe 
point. 
c. Relation tooxygen; under mica plate; in hydrogen. 
d. Relation to light; (Buchner’s Experiment XLVI.). 
e. Relation to antiseptics and disinfectants. 
f. Pigment production; relation of development to oxygen; relation of de- 
velopment to character of medium; changes produced by alkali and acid; solubility; spec- 
trum analysis. 
g. Gas production; rate, quantity and formula produced on glucose, lactose, 
and saccharose media. 
