60 General Bacteriology. 
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h. Acid and alkali production; carbohydrates present; carbohydrates absent. 
i. Relation of growth to acidity and alkalinity of medium; growth in 1.5, 3 
and 4 % alkali; growth in 1.5, 8, 4 and 5 % acid. 
. Reduction of nitrates; to nitrites; to ammonia. 
. Production of sulphuretted hydrogen. 
- Production of indol. 
. Enzyme production; proteolytic; diastatic. 
. Characteristic odor. 
Pathogenesis: 
1. Modes of inoculation by which its pathogenic properties are demonstrated. 
2. Quantity of material required. 
3. Duration of the disease and its symptoms. 
4. Lesions produced and the distribution of the bacteria in the inoculated 
animals. 
5. Which animals are susceptible and which are immune. 
6. Variations in virulence and the probable causes to which they are due. 
7. Detection of toxic or immunizing products of growth. 
8. Widal test. 
9. Pfeiffer’s phenomenon. 
eo 8 Bare. 
REFERENCES: Chester, Report Delaware Experiment Station, 1897; A. 216; P. B. 
C. (Cheesman’s Charts). 
CLASSIFICATION OF BACTERIA. (MIGULA.) 
I. Cells globose in a free state, not elongated 
in any direction before divisions in 1, 
2,or 3 planes. CoccacEkaAE ZOPH emend. Mia. 
A. Cells without organs of motion. 
a. Division in one plane, - - 1. Streptococcus BILLROTH. 
b. Division in two planes, - - 2. Micrococcus (HALLIER) COHN. 
c. Division in three planes, - - 3. Sarcina Goodsir. 
B. Cells with organs of motion. 
a. Division in two planes, - - 4. Planococcus Miguua. 
b. Division in three planes, - - . Planosarcina Miqauua. 
II. Cells cylindrical, longer or shorter, and only di- 
vided in one plane, and elongated to twice the 
normal length before the division. 
(1) Cells straight, rod-shaped without sheath, 
non-motile by means of flagella. BaCTERIACEAE MIGULA. 
A. Cells without organs of motion, — - 6. Bacterium ENRENB. 
B- Cells with organs of motion (flagella). 
a. Flagella distributed over the whole 
oO 
body, - - - - 7. Bacillus Coun. 
b. Flagella polar, - 2 - 8. Pseudomonas Miguua. 
(2) Cells crooked, without sheath. SPIRILLACEAE MIGULA. 
A. Cells rigid, not snake-like or flexuous. 
