EXERCISE LXII. 
BACTERIUM PHOSPHORESCENS Fischer. 
GENERAL CONSIDERATIONS. Described by Fischer in 1887 (Zeitschrift fir Hygiene, Band II, p. 92). Found in Kiel harbor, dead 
sea fish, oysters and occasionally on meat in shops. The production of light isshown in the dark, especially when the organism jy 
grown on a medium made by boiling two salt herrings in a liter of water, adding 100 gms. of gelatin to the filtrate without neutraliza, 
tion, tubing and then sterilizing (Lehmann). Phosphorescence can even be restored to attenuated cultures by growth on this Medium, 
Inasmuch as oxygen is necessary to light production surface growths are best. 
REFERENOES. Lafar 160-164. 
MORPHOLOGICAL CHARACTERS. 
Sultures. 
Age of 
Incubation 
temp. (°C.) 
SKETCHES, 
1, Form: 
a. Bouillon... 
d. Other media 
6 POs cnc navasns 
. Cell groupings 
and arrangements :2 39% ctstanaseme iene ve ce seteeinee memeuuies teed bai iasaQieineaebbchnetate aeacage)S ane Aoeged eC ues Ga chance elveraneein Macrae ees 
a, Aqueous gentian-violet..... 
5. Loeffler’s methylen-blue.... . 
c. Gram’s stain 
d. Special stains 
OMA GAIA 92 cee cM ON ea NN Oh ae A A Sa set DOO Sass ese ae AT Ae NA Teer thE ed se teria as 
a. Character of movement 
6. Flagella stain 
SOR a ceeded 5 oie vance ded a biSiuahges ay 1s Se Satara wie Sides acd aE athe @aentd etched ne DRA e RIAN ey aaa Rien ante mss Ege 
desiccation, light, disinfectants, etestii. sis iwns cnisisdarsaeeneiae neley epinsiaasinweaverd’s Gs £6 tetas nedvse meee gout 
4. Pigment production:...... 0... .c0. cee cette eee EEE eee nee ca ee cone EE EE Stuy ctbE bebe dEE bEbE bbeL bebe bebe babe babe bebbibee cbebuansat " 
g Gapedueeaminemneeda?, ==CU~C~C~‘“‘C‘“‘C“‘(Sé~*C*‘“(‘ a SO 
@, Shake culture .. 20.0... cee eee cee eee ee EEE EEE EERE EEE EEE DEE EEE be bbe bel 
5. Fermentation tube, growth in: (x) open arm... ..eccceceeecesecceeceee cesveeee(@) 10800 AIM! ceecsoerecee 
(3) rate of development: 24 hours ......,........ per cent., 48 hours .......... percent., 72 hours ........, seee POL Cent.) . consis cad 
fa) weacilot It OPE APMG, .0.icincensi ater oxen nasrunwed nus goeenien aa v++00(5) gas formula, H :CO,:: 
6. Acid or alkali production, litmus milk. .... 1. ..1e see ee cece eee eee eee eet e cede ceae tenet end crite dabei bebe bbe ce 
7. Reduction of iti Ps tea atta aay ane cdcrgcercnad ah eh dnaain ae daa tah en Noein mae natn. ae cree tees 
8. Indo] production; 24 hours ........-.60. cee eee eee te eee 6 ABR OUES scsars davsins winded vase Mae weboeenpee ina a 
fecal odor; 24 HOUTS .......66 06 ce eee eee econ een eens AB NOUTS (5:5 seers alsin? sdayownn al Seu hoy odcees 
9. Enzyme production : proteolytic ........ 0... cece cece eee eee ener nsec ners lets veceucens 
10, Characteristic Odor... .... 2.2.6 e cece cee eee ee ee ee ee eects neE tebe cite sien bee eens 
11, Pathogenesis .........-6..scceeeee eens cere eee eee nee een tnee tbl bte ibe cbae eee. 
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