Medical Bacteriology. 89 
(1) By heating to 60-65° C. for 1 hour on 5 successive days, and finally plac- 
ing the tubes in a sloped position in inspissator (or sloping tray ina high temperature in- 
cubator or steamer) and heated above the coagulating point of the serum (70° C.) In this 
method the clear serum is used and not Loeffler’s mixture and a transparent medium ob- 
tained. This method is not usually employed, but the following: 
(2) Loeffler’s mixture is used and the tubes are immediately placed in a sloping 
position in an inspissator, or steamer and heated up to 95° ©. for 1 hour on three con- 
secutive days. If a higher temperature is employed bubbles are formed which rupture 
the surface of the medium in their escape. When sterile the tubes should be sealed with 
paraffin or otherwise. 
REFERENCES. A. 106; H. 45; L. & K. 88; M. & R. 50; M. & W. 81; McF. 181; 
N. 463; P. 219; S. 87 & 35. 
