Medical Bacteriology. 99 
The Micrococcus gonorrhoeae does not grow on the ordinary artificial media but may 
be cultivated on the following: 
a. Blood agar. Blood drawn from the finger, under aseptic precautions, in a cap- 
illary pipette is placed on the surface of agar either in tube or Petri dish. This blood 
is then inoculated with the material containing the organism (pus or pure culture) and 
smeared over the surface of the agar either with the loop or better by means of asterile 
eamel’s hair brush. : 
b. Wertheim’s method. Human blood-serum (from placenta or pleuritic or other 
effusion may be used) in a fluid and sterile condition is placed in two or three test-tubes. 
These are heated to 40° C. and inoculated with the material containing the organism, 
making dilutions from one to another if necessary. To each tube is then added an 
equal quantity of nutrient (ordinary or 2%) agar thoroughly liquefied and cooled to 
40° C. The two are then thoroughly mixed and quickly poured into Petri dishes and 
placed in the incubator at 88° C. Colonies appear in 24 hours. 
¢. Rabbit blood-seram may be used either in a fluid or solid condition. 
