EXERCISE LXXXI. 
MICROCOCCUS TETRAGENUS. 
SARCINA TETRAGENA (Gaffky) Mig. 
First described in 1883 by Gaffky. It is found in phthisical cavities and sputum and it occasionally occurs in mixed infections gs 
abscesses connected with carious teeth, about the neck, jaws, and middle ear, rarely elsewhere. 
REFERENCES. Gaffky: Langenbeck’s Archey, 1883, 28:500. A. 309; H. 189; M. & R. 171; M. & W. 
133; McF. 
448; P. 472;8. 314, 
MORPHOLOGICAL CHARACTERS. 
1. Form: 
Bs Bou OM sense arya Cera ie ty eet ealerigtege TY Sa BO a eee 
Oe ABT ene 2a eras PEAY ee ee Ae RED Laie ieie Se are a heen lp tog aNd aia tas |ticaa ale esl Acar os so adhe i ORE TEA core me NejoueTy 
G2 (Gelatin in on eetecacew tana Men CaN SS RNAS APS 
d. Other media...... 
PARISI Saran ene aeh nr heieee ire trae Ure te eae oe ae yore ea vane 
9s Cell arouip tg Sica ayeyeieis ace nespeanesaia tie asia ave etn se eee mS Sade SE om ae 9k a 
ANGATTANGEMEN tS: sane cu ecace ve ae emits aes qcitagy em ebiRa Maids ne meminen cea aeioeerete Ra GATaELEs HE ARR MEO oe ORME te eet a Oe 
iM OFOWENS: ears doee spikes i dead Mee donee 
os 
a. Aqueous gentian-violet ........ 
b. Loeffler’s-methylenzbl tie’ os sicscscsece acta . «class we sdinoma. vie os) anna we aatha ane Winans Sig eS EG Se eating, @BeOAE gh aaagdE eae eats 
Gs GRAMS: Stains ss ska case Se nt Gags habe oe ak Goss Face ae te Bs eee dd ene ea o we at Ea ems or eae eT 
@Special:Stains$*s wicaads ese ugncus newadeunecadeae 
J OLalMINg POWSIUSs: \ekeaG weigh 16 Aba dots eee eran Trib Sidadisstt eee dl arb senha Ad Saas ates Dae, ae eee Re Ae rgey pore. deel apts AUR EAE ATA ee 
b:-Flagellastaim sacie vic quan kn Mewewenn wae meus eda 4 oe alanine 1h ae ES GMpe mena eatin oe on de apes 
GO DOES Ara yiinna een Re ere a Re eh ean Osea oe Rak cea Sieh nates ern ean SANG 
7: SpeGial Characters) sSUCM AS i i.ve.geqarrmne ne eansen, selnereiens wuadhe ne eM aRoRE sta Ana Manna MeOne aa daa ea SaROa an ae Aas Wan OS nies 
deposits, vacuoles............. 
pleomorphic and involution forms, capsules, etc 
PHYSIOLOGICAL CHARACTERS. 
” 
2. Relation to free oxygen:............. 
3. Relation to otheragents)jsueli Asie a: ese taaeeae. avin sade SRARe Se Wiel eae eas g alma Ae aie eahoas owALs smo Me we ea tis gueeney Sdeces be buen nea eacten: ilo eat 
desiccation, light, disinfectants, etc.:. 
ti Pigment productions... .ciscax sears tts ees ee 
5. Gas production in glucose media: 
a@: Shake culturé .22iccs0 sass cous 
&. Fermentation tube, growth in: (1) open arm 
(3) rate of development: 24 hours................ 
(4) reaction in open arm:.... 
6. Acid or alkali production, litmus milk. ...... 
Reduction of nitrates; to nitrites........ 
NI 
a 
Indol production; 24 hours................ 
fecal odor; 24 hours. 
9. Enzyme production: proteolytic......... 
to, Characteristic odor.. 
In, Pathogenesis. cscs. osc 
Age of 
Cultures. 
Incubation 
temp. (eC.) 
SKETCHEs, 
Relation to ‘temperatures: -2o.sicce oc sc seas oe ae ae yamiea cevisnees 
Fe gaRE Gg RtSETe.N Sus Gueee sald Ae mre Aare (2) sChOSEG) SANE Actin ce Ree wy he Soin de Alias eae ese un tte anes Mem ananeiem 
per cent., 48 hours.... 
seoeee-(5) gasformula, H:CO. :: 
aces: PO MMOUNS Soe es ccasecgncs 
Seare ks WB HOUTSx se sae 5 
[102] 
see per cent.,, 72 hours’... 1... 
ty dee DETICCD Gia sean 3 
wea se MOUTS..c02 
wo... per cent, 
2 36) LOY ATMO MIT Ais 5 sasvd deduce hak wooed ventas 
