EXERCISE LXXXIX. BACTERIUM DIPHTHERIAE (Loeffler) Mig. 
BacILLUs OF DIPHTHERIA; KLEBS-LOEFFLER BACILLUS. 
First described in 1883 by Klebs. First cultivated in 1884 by Loeffler. Found in the false membrane in cases of diphtheria and in 
small numbers in spleen, liver, etc. ; occasionally in healthy throats. 
REFERENCES. Klebs: Verhandl. d. Kongress fuer innere Medizin, 1883, II. Loeffler: Mitth. aus dem Kais. Gesundheitsamte, 1884, 
2; 421. 
A. 349; H. 162; L. & K. 207; M. & R. 353; M. & W. 137; McF. 284; P. 329; 8. 356. 
MORPHOLOGICAL CHARACTERS. 
Cultures. 
Incubation 
temp. (°C.) 
Age of 
SKETCHEs, 
1. Form: 
G, BOuUilOnyscacdent sa te hea Arod tans et BFS COWRA ea ONS 1 OTe Ayes leon’ Get dena we ae, oda eS RR As Eevtisieana rs Seer eras ta: lS e Sarees 
G55 GOLA ELI Scis-chscscna to agerercrsapelbia erase gover eace ae ae abv aes hc aoe gmpeanin als Sie ae Tae so aaNet a tidaye ghd dL RIDE eee eNom ORE Bea aA NR anals. Sb gps [latest 
i OMee medias: ys. cgee sy os seek Bebe ahead a ORE aed ae Ede fa Sees EES et eee a es os epee ares pa cee eat teamed 
iS Ha Oar cie eet ait ale eceae VS gopel ae lesen, DOL a VIO Ne DIN AS 5 LU <ESTCUR VEL uate ce Legh GETS Sac me one Se eM are ASSIA ee eam see or Gress 
3. Cell groupings os essa: se gee aia Some tee ve Hs ERROR RIE sce RG Re ARNE Rein RE NAAA ET RR a LER Roao eee pow euieMe Sanlu es D> GH 
and arrangements ins weve eee enizaoria teh nig oot sinac amended Ha melameals ates 
ITIP EOW ENS scacecetnde ce sehes ao hare AE AUER OE BAR RR Na a bed Sed te aaa SU nee ae adele zaaLeacin nent seme meee UE ea 
4. Staining powers: 
a. Aquéous gentian-violeti sess ecm came canes ue aia aeeN nS HORS ALAS TE SEDI AE Gd wan oald ee OVO aR NY Soe EES 
b,.leoemer’s nrethylen=zblucin sx tas /cncssviaas fas alata aalomiann nah eoualsiae teas Paola he UMMA ce FEAT RE aan Betactn as ne eae eeraS 
6 Grams Stainwenesdse- was eis as acne id ae Seok oeea ant een In ee Pdi ee eee eR RAG Adltn ails ates who saaieise nthe saan meres] ahs aenatinallla aman 
GSS P ECVAIS PALMS 56.52. Sy Aensse esd, J sce cisosch ete ch 2 Sey aca byes depts ese ato ial tude Gh aceasta eeeseiet he ve nN SARIN ARP 4 cave wee AME ah 
Bis MLO UGY ste riecc. ataxia ca rsurpais cays ASSIS Vena Te eG NE AVE WEIN Sena des tales main ACR iA ei etal 5 Culestare aut Wyiealiten sdk ae fechas eens meee NE a 
@. Character-of movement cess sens emecantace ea atena cium 4 a8 dainael sh dann nda Bo SND We my eas GU GS Tura REE Ane Mane aaa 
6. SS PORES, 5s. Fos.5, se seeasshs as ese 3 SS OS Na aiSd GS GS BES Ca a ENE STG Ur Bee RES EB a DEED. Stir erev at dina ye aie ape] ate tes 
5: Special characters, Such Ase sac eewate sce cpacere sictane eater tai ates ae cues arora eee ca ah arm ap tess eee ere NSU HE RG IO Ea bE Eerdae se rain | Resa eaa ibe ee een 
deposits, vacuoles............. 
pleomorphicand! involationfornis; capsules; €tC vies aa hecee ake we eeng Ayes fa inate sa 3 RA re ee ee ng ed Ae eA We 
PHYSIOLOGICAL CHARACTERS. 
Tt, Relation to: temperatures: . 2. scuce sone as sestisaserenag geass 
Ziv TREAT OMCOMTEE ONY REM: Ssh scege si ures a arabe ots Pe Ges hd Ws wi Ad cee eutia se te ancl pian eben ee eT Hek  seanvchotaauseee ale beimeeese acletid ates pc Toreaeaee dete eae ed eee clube re ome openssl stove alemiorma eet 
3. drelation: tooth emarents Such, asians cans sae Gani Zagato anyedta iy anahkas anise a athe scl uel antag ne eunlome wap ge Beate yeh dia cs unt gotnaute aerial eh ale 8 hace anil dag Mee 
desiccation, light, disinfectants, etc.:......... 
4e- PIP MEN tp Od UGLO IAs ook wind te edione Avot AneR My RE AatLAAOR SAE ENA @ MRL ces Ue else Soles i dan od Sued aps a shawrerme rang hhc ne Geucane 
5. Gas production in glucose media: 
@: Shake cultures. a2 Gacscey sense debieaes audi ae ge cule 
&. Fermentation tube, growth in: (1) 
(3) 
(4) 
open arm:. 
rate of development: 24 hours................ 
Faction M-OpeNalMgwacieioces tate se caus shveeeas anes dzae wee guess abs 
sposiien (2) Closed ATMA uae avs ge aly von Mowe ona set dundied Huds amma rioetae amen ane HMR 
per cent., 48 hours............percent., 72 hours.............. DEF CONtiy cs ssess seas 
......(5) gasformula, H: CO, :: 
6. Acid of alkali productiom, [itimusiinilkey vo. naawee wae geek heen ate de Fee Mena a4 Vatyea iva adiwernaes 
7. Reduction of nitrates; to nitrites........ 
8, Indel productions 24 hourtsij.2c secede ase aa ea ieee naan 
feCal“ddor: Se NOUTSsuswed cas cae Hea dcwmmiey 
g. Enzyme production: proteolytic.................0...... 
To 0 ChiapactenStic- Od Omscsnweve tase orate fey 
peg P Sede scar dwar tes Bac ee se Methane wen deere ew 
AB SNOUTS UCAS Ge hese enim eee aay 
puis CAS LAN CHicarstraiaky da, caste hene conte 
ri,. Pathogenesisi..9; adistet Gate Mchat eat inereancz a 
[118] 
ages cae ROULS ace 
. se... per cent. 
Foam Ona. 2 3: Noss atestnnst. ne eaten Ss Beenie emlau wemsmatia a pan aanamanmeina putes eee 
theadee AAYS wasyrasnmedays ye ca ix eeancwneiel aM 
