EXERCISE XCI. 
BACILLUS TYPHOSUS Gaffky. 
BacILLUS OF TYPHOID FEVER; EBERTH’S BACILLUS. 
First described by Eberth in 1880, first cultivated by Gaffky, 1884. It is found in the faeces and urine of typhoid patients. 
REFERENCES. Eberth: Virchow’s Archiv. 1880, 81;58 and 1881, 83; 486. Gaffky: Mitth. aus dem Kais. Gesundheitsamte, 1ggq 9. 
B72; A. 
369; H. 223; L. & K. 166; M. & R. 317; M. & W. 141; McF. 366; P. 402; 8. 337. 
MoRPHOLOGICAL CHARACTERS. 
Age of 
Cultures. 
Incubation 
temp. (°C.) 
SKETCHES, 
1. Porm: 
Ms BOUIN GMs nize bse ables Garner 
6. Agar 
CAG CLAW ecnctecamaratetheke uk Sanwa sone enon ant 
da, Other media.... 
De ULC sashes Okey et sae nies 
3. Cel] groupings........ 
and arrangements . 
In GROWtHS \c.uies can ieee Bow Ane Sader ere atee & aa ehs 
Ge Stain POW ETS} de soie yooh cieomno ia gies ae RAGS Ee Ce 
a, Aqueous gentian-violet 
b. Loeffler’s methylen-blue..... 
@, Grain’s Stalin seg cnes nevi wees wees as 
d. Special stains .. 
5a. Motilitytirs cgi ccaees 
a. Character of movement 
6. Flagella stain 
ie SOLES 2nd a ennnracn cate eno ce RIGS We See Wate ete Nea SoA Sa onian eae soo seni aide ge tones 
7, Special characters, suchas: «021.0... -48: 
He BOSIOS: VICUICIES 3.08 ous awneis sea venwomwy as yee nen es eenieeris% wees 
pleomorphic and involution forms, capsules, etc. © ......-.. 0.00025. 
PHYSIOLOGICAL CHARACTERS. 
1. Relation to temperature: ......... 
2. Relation to free oxygen: .............-. 
3. Relation to other agents, such as: 
desiecdtion; light, disintectants; 206%. .0000je wi arti ceeds tensenep maser acids oe ewan 24 aedeloleagy batee be bee 
4. Pigment production:............. _ 
Ree res - 
5. Gas production in glucose media: 
a. Shake culture...... 
b. Fermentation tubé,.growth in: (1) Opemsarm? oc. in coonce ny 25.0 ona Beek ncad ee cae (2) 
(3) rate of development: 24 hours........ veeveee per cent., 48 hours............per cent., 72 hours............ per cent., 
(4) reaction in open arm: ...(5) gas formula, H: COo:: 
6. Acid or alkali production, litmus milk 
7. Reduction of nitrates; to nitrites ...... cose. ese enseiein eee 
8, Indol production; 24 NOWTS «20.25.4024 o2 sees eo a eis oh , 48 hours 
fecal Odore2a HOUTS sc. wa ccnmorcnseeresd een ne ries (onal Gina Seer NN css ie 
9. Enzyme production: proteolytic... neterline tates 
to, Characteristic odor....... 
11. Pathogenesis .... 
GIOSEG a Pini acerca: Vode aenteoign Ha anise : 
& Sep ibO- ANIM ONIA wens le ee A Be 
per cent. 
