EXERCISE XCII. BACILLUS PESTIS Kitasato and Yersin. 
BACILLUS OF BUBONIC PLAGUE. ¢ 
Described at about the same time independently by Kitasato and Yersin in 1894. Found in the buboes, and occasionally in the feaces 
urine and blood and, in the pneumonic form, in the blood. 
REFERENCES. Kitasato: Lancet, 1894, 2;428; Yersin: Ann. del’ Inst. Past., 1894, 8; 662; A. 292; H. 259; L. & K. 200; M, & R, 437. 
McF. 433; P. 606. 
MORPHOLOGICAL CHARACTERS. 
Cultures. 
Incubation 
temp. (°C.) 
Age of 
1, Form: 
Be OWT ca oe Sur sa vencnlene Wpeied ceded 24 Ma eG Geen ew te a we ety Kr oe Ga as red Kewanee ¥ 
DU INGAT si ahcnee es 
SNOUT ae ay cs darrian a ic ee es to ae ain ace Seo GAR Men ee a Hs PONS GHEY AE GE GUT 4S GOOSEN TE HATE 
d, Other medias. sc cciccccaccs: 
Bie SUZ Ow son ereocpenin ne Annie a Ah cree late NAA a tap rolep Nk tale Aten Ree ae lcci ta Thane Lanier had eal ater 
3 Cell BLOUPINES ashes cecke rs hs whe taming tren ae aes ear asaya GA ae utah AIR aEe a MAR tinraedun ik WA ered 
ANCVATHANIGEMMENS 3) 37.5 e asain den, G aaah mayne ELIAS LULU fv DGG etapa ease lvotepepeciaa alae cates gs orate - seca os toes ee eR eee 
AME TO WENS see ctcreeeciseigsiins veg Sims wrrcrican wie iehasie arnada ne ako etme eRleela sea SG Ad Heloa as wimenlg Ge ymecealove at etd esata suai ah BE Meme en 
qo Staining POWE Sins sn sacks auton cu uy memes og ends 
a. Aqueous gentian-violet..................... 
B -OCMEer Suet nh Vle d= DIU Cie ce 3 uiadarctiee ato Se ree uss actvale igen ak Siecaeltes eredsicd aed pacpauaes Saree mae ewe mele 
EMGTATI S| STAIR a aon «knots ane nace mene eenetenes oan ieee aneeer name 
d. Special stains .............. 
5: Motility: vss ex cneseia nes 
@:, Chara Cher Ol MOVE Mie ME se arash carers Moa aaa soe har acAl Cog ca ae es dtc ih GPs tetha Hit sg ma ete Pah weer vocals creche hems ans testes etalon he 
OI SPOLESS assis skaie ohrsrnaenns ono 
7. Special characters, such as:...........-... cece eeee 
GEPOSITS WACUOlES mache AM agiias om ARAL Se Toei Set ead nec eee eel pte day 
pleomorphic and involution forms, capsules, etc............... 
es | 
SKETCHES, 
PHYSIOLOGICAL CHARACTERS. 
t.~ Relation fo températuresiccas os nce an Ghat saaatanoatan 
2; Relation to:free oxy Pens c6 oscar can czinoans sees exec 
5. Relation to otheragents, such ast svc ov ye nd bites sae ea ssn Gare Se ean Svea 
desiccation, light, disinfectants, etc.:..........0.... 0. 
4. Pigment productioniza. ccc. usceaeseis seas cada Hee awe 
5. Gas production in glucose media: 
a. Shake culture ...... 
é. Fermentation tube, growth in: (1) open arm:.... 
(3) rate of development: 24 hours ............... 
(4) reaction in open arm:................. 
6. Acid or alkali production, litmus milk. 2... ......... 
7. Reduction of nitrates; to mtrites........ 0............. 
8. Indo] production; 24 hours 
fecal odor; 24 hours ............... meee ta ae 
g. Enzyme production : proteolytic .................... 
tO; ‘Characteristic: odotws< a: ix cacneaimotenese aan 
ir. Pathogenesis ........ 
per cent., 48 hours .... 
Jip 4B NOUNS sors Sa hs ares oe ee ireeeae oo lhok onsen 
eek WO WNOULB acerd pricey esd bps) Mid ee ans 
+++. per cent., 72 hours ........ 
sees... (5) gas formula, 
++...) to ammonia............. 
occ hours... 00.06. per cemt, 
