EXERCISE XCVI. MICROSPIRA COMMA (Koch) Schroeter. 
COMMA BACILLUS; CHOLERA VIBRIO. 
First described by Koch in 1884. Found in the intestinal contents of cholera patients and has also been isolated several times from 
a water supply. 
REFERENCES. Koch: Berl. Klin. Wochenschr. 1884, no. 31 u. 82: A. 401; H. 244; L. & K. 181; M. & R. 402; M. & W. 152; McF, 311: 
P. 568; S500, 
SKETCHES, 
e of 
Cultures. 
Incubation 
temp. (°C.) 
MorpPHOLOGICAL CHARACTERS. 
Age 
1. Form: 
iG BOWilOiy was cg cc soe yeas OS See ae he ee Pa Be Oa Ba a eed B eSina een eee dd gt CaS Glee Bee Ga eenin ease ametinnl couse. | eee tate 
Oi INE 4 cacteteeieie, os aisha! seatey tao anbeana ee igh dlab aA Gaba RRIpRG NE Eetetie Andante tex SNERSAIN aa apendanaeht ohana ies: paslchalleA sas dentine alee la dai gos Be Rll area tnten PRONE TY 
Gra Gy CUAILING, Se oi haart Renteare i ea tenc Bossy ue eants Seo eee Magis De kara eee se ae sate aman Se ATE Oi ahene He Shiva raae ee Sethu en oe aoe RS ene BEY ainte | ebalgtaa tell oases, teers 
d, Other media........ sogcad ae ssyclaanacheg dete awensasie/elalabrornict ens nad akotarata hedslederaustewa otne. ev nah «i PeaveNslanvie Sbeuesayes vy ara ceamcorei@ cneoulntbaane aah hie rh deere lg eave wets | ones peer 
Be SIPC wea ath Bg fG aA ge eae Bo Send aw Gaede be bee eteN whereie De aecaicermie se Re ey eth ee tees om Bi etic Sodiart eee anaes ae bach ae 
ANG LAT PAMMEMENES sy5:3 ce wes ca ssralind Mee eA PEELE eR RAD AG BA Hees Mamet eae SNe ae Hoos amNnS 7AeR RAS Wa wean |anoen as Hallsen cen 
THER OWENS Art ar clo elae es Rie laa, aie sloshed on iereneevnstels La iseuclaiae emits 
s SLAM. POWETS! a4. as Ghee sraneet se wa xen ese oay 
= 
a. Aqueous gentian-violet......... 
Ox Thoefilerscnmveth yle ac DIl es seve hic aon ae ett et acs ersutee aaatayannabarsyeieptadia laa tebeabie im a apn weaieaoea rebates oe asad ae esa aisle Ieee 
@; Special’stains|: 2s cecissa de oeebess 
oped) Caviar pins Agree eee ae is rari CONC Ue erent CHE ce ee nerds eri tures Tee Oe nC ani err ey at Mean ae eeeargre ee ee AE ACRE Oe ee ae ere 
wa 
a, Character of movement ....0.0.. 066.60. cee 
bs PlaeellasSta nna sams cas tacen ar aura celeines emacs ake ras Peaasereheacnee 
6; SPOreSies cerns 
. Special characters, such as:........... 
NI 
deposits, vacuoles .... 
pleomorphic and involution forms, capsules, etc.. 
PHYSIOLOGICAL CHARACTERS. 
i: Relahionecto, temperature se... sesccss opens se neneeta) asieewiendase vaca ee 
2. Relation to free oxyrens, vs j...e op ee kann 
s. Relation to other agents, such as:............ 
desiccation; light, disinfectants, etev.c cocci vesagaeccie vase cans 
4. Pigment production:.............. 
5. Gas production in glucose media: 
a. Shake culture ...... 
6. Fermentation tube, growth in: (1) openarm:............0........0000... ses... (2) Closed arm: 
(3) rate of development: 24 hours ............... per cent., 48 hours 
RAE per cent., 72 hours ............. percent., .............. hours per cent, 
Qh) TEACHOMUM OPE ADM... nis ss tenses cca were ee tengigle de aacieaokamonnea(§) ‘gasfornula, Hi + COg:: : i 
6. Acid or alkali production, Jitmus milk. .... ..0........ 
ao RedwetroiGl rintratess toaitritesi in wasce cha rcesg ewns area ean eo oats emumet aw clases iste, OO BAMIIG NLA a7 ah 45 ie ok od meaivee A amaaye nooale 
8. Indo] production; 24 hours ......... ; Bis aol eerie GH OM MIOMIT Sea 4 <3 pads cichs Se urahe Me AINE anes catwikr Sashes Sita days vad 
RECA OH OTS SH TOUTS oc pada rtd wt oe obey AON we ete ae GO OUTS ucinman ee meta ued desan cnc BaeretosAt eee ts days oe | 
QO: Enzyire: production 2 prove ov ytie: wees sc acesveteneate ea ee ae ies aise dees eybri a tuectierareantCl TAS CALI Cigna wend renee Ue aetna ee 
10. Characteristic odor..... ee Teese antes cues satan ily 
11. Pathogenesis 
