EXERCISE CII. BACILLUS TETANI Nicolaier. 
Discovered by Nicolaier, 1884. First cultivated by Kitasato, 1889. Bocuse in man and animals suffering from the disease and wide 
distributed in nature, especially in soil. 
REFERENCES. Nicolaier: Deutszche Med. Wochenschrift, 1884; Kitasato: Deutsche Med. Wochenschrift, 1889; A. 469; H. 9 
L. & K. 230; M. &R. 376; M. & W.171; McF. 274; P. 385; S. 482. 
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33 3 B SKETCHES, 
MorPHOLOGICAL CHARACTERS. &S gs 
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1. Form: 
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PHYSIOLOGICAL CHARACTERS. 
Relation to temperature : 
2. Relation to: freesoxy gems cn. casas sive etal iy da yalalgnaea resets euaed vaca meray ua siduadc we Sv andi glenda babaetinthe 
3. Relation to other agents, such aS:............ 0... ccc ccee cece cece ceaecnaeuee suctnets ine (NGL Was (aterage teeansisilaiw Vidigtaleteue Meaq AMAR SENS AHEM wij dunvalaiesibcuags bd vigeheed SBA4N decks ye Ne Mea weds Ga daeealenas 
deStccation, light, ina I EEIIDES, CLC 2s ot copes a hans Seiad aawisien ot de Weenies 
4. Pigment production: ... 0.20... ..esese seen cece cece ee eee eee eee cee en ete eee cena een eee beet sean Geese see scae cube tcdataee ests eee bEes cobb tb be bebe ceecbee Gecueee ivi 
5. Gas production in glucose media: 
a. Shake culture ........... iis ase Se Tagine eeaiead sera aes onl hd laceaiesecaiciaeetaver Satins eaeiegnatnes iGtes aed meGele een? Sa Ae aia Nol eae ADE as Shae Sea lesaigy ate Ravsteth SRO 
6. Fermentation tube, growth in: CE) OPEN ALINE eee eee eee eee eee eee cee eee se (2) Closed arm: oo... ec cee cece cane ce 
(3) rate of development: 24 hours ............... percent., 48 hours ......... percent., 72 hours ............. BEF COL, ose cane sevnes ROBIE an wos ve per cel 
(4)  POACEON HI OPEN BLING casas senaccsesssecen ines can : --(5) gasformula, H:CO,:: 
6. Acid or alkali production, Jitmus milk. .................. 7 
ve Reduction of nitrates, tormitrites ic. css sigan vovanad ia bs doewRte sa ainne ad oe navemnwanmans » to ammonia........ 6. eee 
8. Indo] production; 24 hours ................00- ietdba “eee Sh OSDOUFS cas excite sessed aeee aaaks weed ce Fi cusihea: es fein hedale ais Mime extant te days 
fecal Odor: 24 HOWE: 25 ssi ne se owe seater ned acme seals y ABOUTS wines verncies yds dkios cieiocaacaech yy WE Rete a-ak gaat mice ee GAYS visasiasea de valsaanead: ves vetees 
9. Enzyme production: proteolytic: .... .6scisescans cus os oy deeeeereadeesseesvevcoenucueecves GitstatiCwnsanwesiiva dn nesiasacaereocame ibis STOR we nekoishs AIS Het Fiesta 
to. Characteristic odor... 00.1... i eine eee eee ete eet ete ete cece bene eeee dana session setsueee sear teteuubeteitenererse. eee ee tat scenes 
11. Pathogenesis .... 00.0.2. ceee eee ee eee eee cient eee eee ete ceed cee cu ee seueueee sues teteeperceseceneeees BA Ea crac sg ce 
