176 ‘Medical Bacteriology. 
d. Wash thoroughly in water. 
e. Dehydrate in absolute alcohol. 
jf. Clear in xylene and mount. 
Quick Method. 
. Stain in carbol-methylen blue 10-30 seconds. 
. Wash in distilled water. . 
Tannic acid solution (10 %) 4-1 minute. 
. Counter-stain with a weak solution of eosin until sections are red. 
Wash in water until pink. : 
Dehydrate in absolute alcohol. 
. Clear in xylene and mount. 
Bacterium diphtheriae. Loeffler’s or better Weigert’s method. 
Bacillus typhosus. 
. Loeffler’s methylen blue or carbol-fuchsin 15 min.—24 hrs. 
. Wash slightly in distilled water. 
Place in 10% solution of tannic acid for 10-60 min. 
. Dehydrate rapidly in alcohol. 
Clear in xylene. 
Examine. 
. Mount in balsam. 
Such sections examined under a low power will be found to contain heavily stained 
masses, which under a high power prove to be clumps of bacilli. Not infrequently the 
bacilli are difficult to detect in tissue from typhoid cadavers. 
Bacillus suipestifer. Loeffler’s method. 
Bacterium Welchii. Weigert’s and Loeffler’s methods. 
Bacillus chauvaei. Use Pfeiffler’s stain: 
a. Dilute carbol-fuchsin % hour. 
b. Absolute alcohol slightly acidutated with acetic acid until section is a reddish 
violet tint. : 
c. Xylene and examine. 
d. Mount in balsam. 
Bacillus oedematis. Pfeiffer’s stain. 
Streptothria actinomyces. 
. Ziehl’s carbol-fuchsin, 10 minutes. 
. Wash in distilled water. 
Picrie acid (cons. ale. solution). 
. Wash in distilled water. 
Wash in alcohol (50%). 
Dehydrate in absolute alcohol. 
. Clear in xylene. 
. Balsam. 
Tissue stained yellow, rays red. 
REFERENCES. M. & W. 2389-286; N. 537. 
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