192 Medical Bacteriology. 
ically and by means of plates. Then test the peptone cultures for nitroso-indol (cholera 
red reaction) by the addition of a few drops of sulphuric acid. 
BACTERIUM TUBERCULOSIS. This organism has been found in the stools in cases 
of intestinal ulcerations, and may come, in cases of phthisis, from ingested sputa. 
AMOEBA COLI. 
1. A drop of the mucous portions of stool is placed on a glass slide, covered with a 
cover-glass and examined with a magnification of about 500 diameters (4 in. objective). 
Examination should be conducted on a warm stage in order to get amoeboid movements. 
2. Preparations may be stained with methylen blue and carmine. The nucleus 
is stained with the carmine. 
3. Discharge may be hardened and stained by Mallory’s method as follows: 
a. Fix tissues in alcohol. 
b. Stain (paraffin) sections in a saturated aqueous solution, of thionin for 5-20 
minutes. : 
c. Wash in water. 
d. Differentiate in a 2% aqueous solution of oxalic acid 4-1 minute. 
e. Wash in water. 
f. Dehydrate in aleohol (95%). 
g- Clear in oil of bergamot. 
h. Wash with xylene and mount in balsam. 
Nuclei of Amoebae brownish red, other nuclei blue. 
REFERENCES. v. J. 199; Si. 228. See also texts under various organisms. 
EXERCISE CX. EXAMINATION OF URINE. 
For bacterial examination urine should be drawn with a sterile catheter into a sterile 
bottle. 
BACTERIUM TUBERCULOSIS. 
For method of staining see under Sputum, CVII. 
It is best to centrifuge the product and care must be taken to differentiate from the 
Smegma bacterium. or this purpose stain cover-glass smears as follows (Bunge & 
Franteroth.): 
1) Absolute aleohol, 3 hours. 
2) Chromic acid, 15 minutes. 
3) Stain in hot carbol-fuchsin. 
4) Decolorize in sulphuric acid (25%) 2-3 minutes. 
5) Counter-stain with a saturated alcoholic solution of methylen blue. 
The smegma bacillus is decolorized by this method. 
Tubercle bacterium in urine is frequently present in clusters while the smegma 
bacterium occurs singly. Injection of guinea pigs, smegma bacillus is non-pathogenic. 
The following organisms have also been found in the urine. For methods of isolation 
see references. 
PYROGENIC MIcROcoccI.. CXI. 
M. GONORRHOEAE. CXI. 
B. TypHosus. CIX. 
S. OBERMEIERI. CVIII. 
REFERENCES. v. J. 273; Si. 504. and texts under the various organisms. 
