198 Medical Bacteriology. 
B. OEDEMATIS. 
a. Make cover-glass ee paratows from fluid of affected parts. 
b. Also make anaerobic cultures. If material contains spores it should be heated 
to 80° ©. for 10 minutes before it is seeded. 
B. TETANI. 
a. Make cover-glass preparation from pus and search for drumstick bacillus. 
b. Make cultures in glucose bouillon and agar-plates and develop in hydrogen. 
c. Inoculate animals with the discharge, and also with the bouillon culture, and 
- watch for characteristic symptoms. 
S. ACTINOMYCES. 
a. Place one of the minute sulphur yellow nodules in a drop of glycerine on a glass 
slide and then apply gentle pressure. 
b. Even the low powers of a compound microscope will then show something of the 
clustered arrangement which can be more carefully studied under a higher power. 
ce. Intraperitoneal inoculation of guinea pig. One month later nodules on peritoneum. 
AMOEBA COLI. CIX. 
REFERENCES. v. J. 405; Si. 514 and 518. See also texts under the various or- 
ganisms. 
EXERCISE CXII. DIAGNOSIS OF RABIES. 
a. The medulla of the suspected animal is removed under aseptic precautions, as 
soon as possible after death. In case the animal is some distance from the laboratory it 
is best to cut off the head, pack it in ice and ship by express. 
b. Place a piece of the medulla about the size of a pea, in 4 or 5cc. of sterile bouillon 
and thoroughly grind up the same. 
c. Anaesthetize a rabbit with ether, clip the hair from between the eyes and ears 
and disinfect with a carbolic acid solution. 
d. Make a longitudinal incision through the skin and subcutaneous tissue along 
the median line, while a crucial incision is made through the periosteum on one side of 
median line thus avoiding haemorrhage from the longitudinal sinus. The periosteum is 
then pushed back and a disc of the skull (3 inch in diameter) removed with a trephine 
and the dura mater exposed. 
e. With a sterile hypodermic syringe introduce 2 or 3 drops of the suspension of 
medulla beneath the dura mater, stitch the skin, disinfect, dry and seal the wound with 
collodion. 
‘The rabbits apparently experience no inconvenience; the wound heals rapidly and 
the rabid symptoms appear in from 15 to 30 days, although sometimes they may occur 
earlier or much later. 
EXERCISE CXIII. EXAMINATION OF MATERIAL FROM HUSAN AUTOPSIES. 
At human autopsies smears from the organs should be made on cover-glasses and 
afterwards stained and examined. Plate-cultures should also be made from the various 
organs or instead parallel streaks over blood serum, agar-slopes or agar-plates. In all 
cases the surface from which the material is to be obtained should first be burned to 
avoid infection of cultures with extraneous germs. Portions of the various organs 
should also be preserved and hardened in alcohol. 
