50 OMAPTKK V. 



vol. ; a similar solution of picric acid^ 1 vol. ; disfcilled 

 water 2 vols. Embryos may be left in it for twelve hours 

 washed for two hours in water, and brought into weak 

 alcohol. 



Mann's {op. cit., xi, 1895, p. 4-80).— 1 per cent, of picric acid with or 

 without 1 per cent, of tannin in a saturated solution of sublimate m 

 normal salt solution. 



The same author's Alcoholic Picro-sublimate (Aiiat. Anz. ,\in, 18I>3. 

 pp. 441 — 143) consists of ab,3olute alcohol 100 c.c, picric acid 4 grms., 

 sublimate 15 grms., tannin 6 to 8 grms. The tannin is added in order 

 to prevent excessive hardening. 



Pacaut (Ai-ch. Aiiat. Mici:, viii, 1908, p. 438). takes 200 parts of 

 saturated solution of sublimate and picric acid, 6 of 3 per cent, platinum 

 chloride, and 5 of IGr^ per cent, solution of chromic acid. 



0. vom Rath [Anat. Anz., xi, 1895, p. 268) takes cold 

 saturated solution of picric acid, 1 part ; hot saturated 

 solution of sublimate, 1 jDart ; glacial acetic acid, 4 to 1 per 

 cent. Also the same with the addition of 10 per cent, of 2 

 per cent, osmic acid solution. 



Fish (Trans. Amer. BIkr. Soc, xvii, 1896, p. 143) takes 1 litre of 

 water, 1 g. picric acid, 5 g. sublimate, and 10 g. acetic acid. 



Lenhossek {lutein. Moaatsclu: Anat. PItys., xxiv, 1907, p. 293) takes 

 saturated sublimate 75 c.c, acetic acid 5, 50 per cent, alcohol 25, and 

 picric acid to saturation. 



71. Osmio-sublimate Mixtures. — Mann's (^Zeit. ici.^s. Mile, xi, 

 1894<, p. 481) consists of a freshly prepared mixture of equal 

 parts of 1 per cent, osmic acid solution and saturated 

 solution of sublimate in normal salt solution (for nerve- 

 centi-es) . 



Detjnbe's [Jena. Zeit. Naturw., xxviii, 1894, p. 294) con- 

 sists of 1 part of 1 per cent, osmic acid solution added to 20 

 parts of a solution of 5 per cent, each of sublimate and 

 glacial acetic acid in water. 



0. vom Rate's, see last §. 



72. Chromo-sublimate. — Lo Bianco [Mitth. Zool. 8Ud. 

 Neapel, ix, 3, 1890, p. 443). Concentrated sublimate solu- 

 tion, 100 parts, 1 per cent, chromic acid, 50 parts. 



Mann {Vei-li. Anat. Ges., 12, 1898, p. 39) takes for nerve-cells equal 

 parts of 5 per cent, sublimate and 5 per cent, chromic acid. 



73. Sublimate and Bichromate. — Z*ikee's Mixture {Miinclientr 



