56 CHAPTER V. 



(The mixture does not keep long, forming ethyl acetate, 

 which precipitates.) 



Isolated ova of Ascaris, even though furnished with a 

 shell, are fixed in twenty-five to thirty seconds. Entire 

 oviducts take about ten minutes. The liquid is therefore 

 one of the most penetrating and rapidly acting of any, if not 

 the most. 



Wash out with alcohol until all traces of odour of the 

 acetic acid have disappeared (I myself wash out with alcohol 

 containing tincture of iodine). I consider this a very fine 

 reagent. 



For Ohlmacher's mixture see § 65. 



MiNGAzziNi's MiXTTJEE {Hicerche Lab. Aiiat. Boma, iii, 189.3, p. 47). — 

 Two vols, saturated aqueous sohition of sublimate, one of absolute 

 alcohol, and one of glacial acetic acid. 



87. Trichlor-aeetic Acid (Holmgeen, A^iat. Hefie, xviii, 1901, H. 

 2).— 6 per cent, solution in water. Fix (nerve-cells) for 8 to 24 hours, 

 wash out with alcohol. See also Heidbnhain, Zeit. iviss. Mihr., xxii, 

 1905, p. o21, and xxv, 1909, p. 405, who makes a mixtirre of 6 per cent, 

 sublimate solution with 2 per cent, of trichlor-aoetic and 1 per cent, of 

 acetic acid, which he calls " Subtriessig." 



88. Triehlor-lactio Acid (Holmgeen, Anat. Anz., xx, 1902, p. 435). — ■ 

 As the last. Gives rise to serious swelling. 



89. Salicylic Acid (Heidenhain, Arch. mil;. Anat., liv, 1899, p. 

 186). — Saturated solution in one-third alcohol. A trial has given me 

 simply atrocious results. 



90. Chloride and Acetate of Copper [Ripart et Petit's Liquid, 

 Caknoy, La Biologie Cellulaire, p. 94). — 



Camphor water (not saturated) . 75 grms. 



Distilled water . . . . 75 „ 



Crj^stallised acetic acid . . .1 grm. 



Acetate of copper . . . 0'30 „ 



Chloride of copper . . . 0"30 „ 



This is a very moderate and delicate fixative, extremely 

 useful for objects that ai-e to be studied in as fresh a state as 

 foasihle in aqueous media. Objects fixed in it stain instan- 

 taneously and perfectly with methyl green. Osmie acid 

 may be added to the liquid to increase the fixing action. 

 For cytological rcftearchp.^ a valuable medium. 



