14 CHAPTER H. 



1 gramme of nicotin in a litre of sea water. The animal is 

 placed in a jar containing half a litre of sea water^ and the 

 solution of nicotin is gradually conducted into it by means 

 of a thread, acting as a syphon^ of such a thickness as to he 

 capable of carrying over the whole of the solntion of nicotin 

 in twenty-four hours. See also Mitth. Zool. Stat. Nea-pel, 

 Bd. ii, 1880, p. 123. 



17. Chloroform may be employed either in the liquid state 

 or in the state of vapour. The animals being extended, a 

 watch-glass containing chloroform may be floated on the 

 surface of the water in which they are contained, and the 

 whole covered with a bell-glass. As soon as they have 

 become insensible they are killed by means of hot sublimate 

 or chromic acid solution plentifully poured on to them. 

 (KoEOTNEPF, Mitth. Zool. Stat. Neapel, v, 1884, p. 233). 



Liquid chloroform is employed by squirting it in small 

 quantities on to the surface of the water containing the 

 animals. A syringe or pipette having a very small orifice, 

 so as to thoroughly pulverise the chloroform, should be 

 employed. Small quantities only should be projected at a 

 time, and the dose should be repeated every five minutes 

 until the animals are anaesthetised. 



I have seen large Medusae very completely anassthetised 

 in extension in an hour or two by this method. Andres 

 finds that it does not succeed with Actiniae, as with them 

 maceration of the tissues supervenes before anassthesia is 

 established. 



Pkeyee {Mitt. Zool. Stat. Neapel, Bd. vii, 1886, p. 27) 

 recommends chloroform water for star-fishes. 



Waddington employs a mixture of equal parts of 1 per 

 cent. sol. of cocaine (or eucain) and saturated sol. of chloro- 

 form in water (sea or fresh), according to the habitat. 



18. Ether and Alcohol may be administered in the same way. 

 Andres has obtained good results with Actinia; by the use 

 of a mixture (invented by Salvatoee lo Bianco) containing 

 20 parts of glycerine, 40 parts of 70 per cent, alcohol, and 

 40 parts of sea water. This mixture should be carefully 

 poured on to the surface of the water containing the 

 animals, and allowed to diffuse quietly throvigh it. Several 

 hours are sometimes necessary for this. 



