66 IJllAPTKU V. 



Mic. Soc, 1898, p. 4ii-!j are in iny view too weak, and sufi'er by the 

 omission of tlie acetic acid. 



MOEBAUX {BM. And., I'MO, p. 2ti.j) talies 15 parts foriuol, 85 of 

 triclilor-acetic acid of 3 per cent., and picric acid to saturation. 



111. Picro-platinic Formol (M. and P. Buuin, Sibl. Anat., 

 1898, f. 2, p. 2).— 



Platinum chloride, 1 per cent. sol. . 20 parts. 

 Picric acidj saturated sol. . . . 2U „ 



Formol 10 „ 



Formic or acetic acid . . . 5 „ 



I find this excellent, but the mixture does not keep more 

 than a day or two. 



BouiN also {Arch. Biol, xvii, 1900, p. 211) simply 

 substitutes formol for the osmic acid in Hebmann's mixture, 

 §45. 



112. Sublimate Formol (M. and P. Bouin, loc. cit.}. — A similar 

 mixture, in whicli sublimate of 1 per cent, is substituted for the plati- 

 num chloride. 



Annt.hp.v formula of the same authors {Arch. Biol., xvii, 1900, p. -11) 

 is 1 part of formol to 3 of saturated aqueous sublimate. Rinse with 

 water and bring into alcohol of 70 per cent. 



Sptjlbb (Eiicycl. mile. Tcclmilc, 1st ed., p. 1280) adds to sublimate of 3 

 per cent, or more 1 per cent, of glacial acetic acid and 10 per cent, of 

 formol. 



Mann {Verh. Anal. Ges., 1898, p. 39) takes for nerve-cells 2g g. sub- 

 limate, 1 g. picric acid, 5 c.c. formol, and 100 c.e. water, or (Methods, 

 etc., p. 97) for all tissues 2i g. sublimate, 20 c.c. formol, and So c.c. water. 



Branca (Joum. Anat. et Phys., xxxv, 1899, p. 7(J7) adds 10 parts of 

 formol and 1 of acetic acid to 60 parts of saturated sohitiou of picric 

 acid in saturated aqueous suljlimate. 



NowAK (Anal. Ann., xx, 1901, p. 211) takes 30 pai'ts of saturated sub- 

 limate, 30 of 1 per cent, chromic acid, 27 of water, 3 of acetic acid, and 

 10 of formalin. 



113. Formol-Miiller. — This is the name given by Oeth 

 {Berl. Mill, ^^uchensc]tr., 1896, No. 13) to a mixture of 1 

 part of formol with 10 of liquid of Miiller (§ 53). It should 

 be freshly made up. Fix for three hours in the stove, or 

 twelve at normal temperature, wash out with running water. 

 Much used, especially for nervous tissues. 



MoELLEB {Zeit. iviss. ZooL, Ixvi, 1899, p. 85) takes 1 vol. 

 of formol and 4 of 3 per cent, bichromate (for the intestine 

 of mammals). 



