FIXING AND HARDENING AGENTS. (il 



Held (ifefe. 8i.lchs. Ges. Wis.i:., xxxi, 1909, p. 196) takes 

 3 per cent. sol. of biclii-omafce with 4 per cent, of formol 

 and 5 per cent, of acetic acid (for inner ear). See also 

 MoEEL and Bassal, Joani. Anat. Phys., xlv, 1909, p. 632. 



114. Chromic Acid Formol. — Lo Bianco fixes marine animals 

 for half to one hour in 10 parts of 1 per cent, chromic 

 acid with 1 of formol and 9 of sea- water, and passes 

 into graded alcohols. 



Maechoux (from Pekez, Arch. Zool. Exper., v, 1910, 

 p. 11) takes 11 parts 1 per cent, chromic acid, 1 of acetic 

 acid, 4 of water, and 16 of formol (added just befoi'e 

 using) . 



115. Copper Formol.— Nelis {Ball. Acad. 8c. Belg., 1899 [1900], 

 p. 726) fixes spinal ganglia for twenty-four hours in 1 litre of 7 ])er cent, 

 formol with 5 c.c. of acetic acid, 20 g. of cupric sulphate, and sublimate 

 to saturation. 



Stappbes {La Cdlule, xxv, 1909, p. 356) used (for Sympoda) a mixture 

 of Gilson's : 100 parts of formol of 5 per cent, with 2 of nitrate of 

 copper. 



Strong {Journ. Comp. Neur., xiii, 1903, p. 296) fixes the head of 

 Acantliias by injecting a mixture of equal parts of formol and 5 per 

 cent, solution of bichromate of copper. 



116. Nitric Acid Formol. — Wilhelmi {Fauna u. Mora Golf. Neiq>e1, 

 xxxii, 1909, p. 15) fixes Triclads in Apathy's mixture of equal parts of 

 6 per cent, nitric acid and 6 per cent, formol, and brings them direct 

 into strong alcohol. 



117. Acetone Formol.— Bing and Elleemann {Arch. Anat. Phys. 

 Phys. Abth., 1901, p. 260) fix medullated nerves in 9 parts of acetone 

 with 1 of formol. 



