SEltlAI, SECTION MOUNTING. 123 



It sometimes happens that the mixture after it has stood 

 for some time becomes turbid, and at last coagulates, pass- 

 ing into a caseous state ; or it may undergo a hyaline 

 coagulation, drying up like amber. But up to the very last 

 it does not in general lose its adhesive properties. I have, 

 however, found it to do so, after keeping for five or six 

 years, so that, to be on the safe side, it may be well to make 

 it up fresh every six months. 



Heidenhain {Zeif. wiss. Mihr., xxii, 1905, p. 331) makes it 

 up with 1 grin, of blood albumen dissolved in 25 c.c. of water, 

 and an equal volume of 50 per cent, alcohol. 



188. The Albumen and Water Method (Henneguy, Journ. de 

 VAnat. et de la Physiol., 1891, p. 398). — A drop of water is 

 spread on a slide painted with Mayer's white-of-egg mixture, 

 the sections are arranged on it, the whole is warmed (?ioi to 

 the melting-point of the paraffin) until the sections flatten 

 out ; the water is then evaporated off at a temperature of 

 about 40 C, and as soon as it has sufficiently disappeared, 

 which at that temperature will be in about ten to fifteen 

 minutes, the slide is further treated as described last §. 



This is a most valuable method. It is quicker than the 

 water method, and, for difficult material, safer. 



See also Ohlmachbe., Journ. Amer. Med. Assoc, April, 1893. 



The so-called " Japanese ' method, attributed to Ikbda by Reinke 

 {Zeit. wifS. Mik., xii, 1895, p. 21), is merely that of Henneguy. 



Mann {Avat. Anz., viii, 1893, p. 442) shakes np white of egg with 

 water, coats slides with it and dries them. He flattens sections on water 

 at 40° C, lifts them out on a prepared slide, and dries for five minutes 

 at 35° 0. 



189. Garlic-water. — Hollande {Arch. d'Anat. Micr., xiii, 1911, p. 

 171) gives the following as more adhesive than albumen : — 50 g. of crushed 

 and chopped , garlic are rubbed up with 80 c.c. of chloroform- water 

 (Codex, A.C.) and filtered after twenty-four hours. Use as albumen. 



190. Schallibaum's Collodion (Arch. mikr. Anat., xxii,1883, p. 565). 

 — One part of collodion shaken up with 3-4 parts of clove or lavender 

 oil. Use as albumen. Sections can be treated with alcohol (not absolute) 

 and divers staining fluids. I do not find it safe for this. Rabl, how- 

 ever (Zeit. wiss. Mile, xi, 1894, p. 170), finds that it is if you take 2 parts 

 of collodion to 3 of clove oil, and make up fresh every four or five days. 



