126 CHAPTER X. 



minute or two, arrange on the slide, and then pour over the 

 sections sulphuric ether vapour, from a bottle partly full of 

 liquid ether. The colloidin will immediately soften and 

 become perfectly transparent. Place the slide in 80 per 

 cent, alcohol, or even directly in 95 per cent, if desired. I 

 have not myself found this method safe. 



Instead of pouring the ether vapour over the slide, it may, 

 of course, be treated with ether vapour in a preparation glass 

 or similar arrangement, which I think preferable. 



Gage {Proc. Amer. Soc. Mic, 1892, p. 82) advises that the 

 slide be one that has been previously coated with a 05 per 

 cent, solution of white of egg and dried; the collodion 

 adheres much more strongly to an albuminised surface. 



Atjbuetin {Aiiat. Anz., xiii, 1897, p. 90) arranges on a clean slide, 

 dehydrates the sections with blotting-paper and treatment with absolute 

 alcohol, then drops on to them a mixtui'e of alcohol and ether which 

 dissolves out the celloidin from the sections, then allows the thin col- 

 lodion thus formed to evaporate into a thin sheet on the slide. Then 

 70 per cent, alcohol and other desired reagents. 



Similarly, Maiee (Miivch. mecl. Wochenschr,, Ivii, 1910, No. 12 ; Ztit. 

 wiss. Mile, xxvii, 1910, p. 385), but adding a treatment for ten to fifteen 

 minutes with sulphide of carbon. 



See also Myees, Arch. Anat. Phys., Anat. Ahth., 1902, p. 371 (com- 

 plicated). 



196. Apathy's Oil of Bergamot Method {Mitth. Zool. Stat. 

 Neapel, 1887, p. 742 ; Zeit. wiss. Mik., v, 1888, pp. 46 and 

 360, and vi, 1889, p. 167). — Cut witli a knife smeared with 

 yellow vaseline and wetted with 95 per cent, alcohol. Float 

 the sections, as cut, on bergamot oil (must be green, must mix 

 perfectly with 90 per cent, alcohol, and must not smell of 

 turpentine), or on carbolxyol {MiJcrotechniJc, p. 176). The 

 sections flatten themselves out on the surface of the oil ; and 

 are then transferred to a slide which (Apathy, Mlkrotechnilc, 

 pp. 127 and 176) has been previously collodionised and 

 dried. 



If the sections are to be stained, the slide after removal 

 of the bergamot oil, by a cigarette paper, is exposed for 

 a few minutes to the vapour of a mixture of ether and 

 alcohol, then brought into 90 per cent, alcohol, and after 

 a quarter of an hour therein may be stained in any fluid 

 that contains 70 per cent, alcohol or more. 



