n^EMATlCIN (hTF.MATOXYI.IN) STAINS. 163 



washing out with alum solution or a weak acid. The splution keeps 

 admirably . 



Rawitz (Leitfaden, 2nd ed., p. 63) takes 1 grm. hsematein, 6 grms. 

 ammonia alum, 200 grms. each of water and glycerin. 



Or {Zeit. iviss. Mile, xxv, 1909, p. 391) 1 grm. haematein, 10 grms. 

 of nitrate of aluminium, 250 grms. each of water and glycerin. 



252. Hansen's Solution (Zool. Atiz., 1895, p. 15S}.— See fourth edition. 

 Hansen oxidises a mixture of alum and hsematoxylin by means of per- 

 manganate of potash. I find it does not keep. See also Maybe in 

 Mitth. Zool. Stat. Neapel, xii, 1896, p. 309, or the Oriindziige, Lee and 

 Mater, 1901, p. 171. 



253. Haeeis's Solution {Micr. Bull., xv, 1898, p. 47 ; Journ. App. 

 Mic, iii, p. 777). — Alum-hsematoxylin solution ripened by addition of 

 mercMic oxide. Mayee {Grwulmge, 1901, p. 171) finds the formula 

 " gives too much hsematein." 



254. Bdhmer's Hsematoxylin {Arch. mih. Anat., iv, 1868, p. 345; 

 Aerzt. Intelligenzbl., Saiern., 1865, p. 382). — Make (a) a solution of 

 hsematox. cryst. 1 part, alcohol (absolute) 12 parts and (b) alum 1 part, 

 water 240. For staining, add two or three drops of a to a watch-glassful 



of B. 



The alcoholic solution of hsematoxylin ought to be old and darlc 

 (§ 237). 



255. Delafleld's Hsematoxylin {Zeit. uiss. Mih., i\, 1885, p. 

 288 ; frequently attributed erroneously to Geknacher or 

 Pbddden). — To 400 c.c. of saturated solution of ammonia- 

 alum (that is about 1 to 11 of water) add 4 grms. of 

 liasmatox. cryst. dissolved in 25 c.c. of strong alcohol. 

 Leave it exposed to the light and air in an unstoppered 

 bottle for three or four days. Filter, and add 100 c.c. of 

 glycerin and 100 c.c. of methylic alcohol (CH^O). Allow 

 the solution to stand (uncorked) until the colour is sufficiently 

 dark, then filter. 



This solution keeps for years. It is well to allow it to 

 ripen for at least two months before using it. 



For staining, enough of the solution should be added to 

 pure water to make a very dilute stain. It is an extremely 

 powerful stain. 



It is still much used. I find that when icell ripened — for 

 years rather tRan months — it is quite a first-class stain. 



BiJTSCHLl {TTnters. iib. mikroscopische Schnnme u. das Protoplasma, 



