ll.KilATKlN (h^EMATOXYLIN) STAINS. 165 



and its effects ; see early editions ; also the criticism of Mayeb (Mitfh. 

 Zool. Stat. Neapel, x, 1891, p. 174), and that of Squirb in his Methods 

 and Formulse, p. 25, and the alternative formulaa of Squibe {loc. cit.) 

 and of VON Wistinghausbn {MMh. Zool. Stat. Neapel, x, 1891, p. 41). 



261. Matee's Heemacalcium {Mitth. Zool. Stat. Neapel, x, 

 1891, p. 182). — HfEinatein (or haemateate of ammonia, §§ 238^ 

 239), 1 grm. ; chloride of aluminium, 1 grin. ; chloride of 

 calcium, 50 grms. ; glacial acetic acid, 10 c.c. (or common 

 acetic aoid, 20 c.c.) ; 70 per cent, alcohol, 600 c.c. Rub up 

 finely together the first two ingredients, add the acid and 

 alcohol, dissolve either cold or with heat ; lastly add the 

 chloride of calcium. 



If the objects stain in too red a tone they may be treated 

 with a solution (of about 2 per cent.) of chloride of 

 aluminium in 70 per cent, alcohol, or with a 0'5 to 1 per 

 cent, solution of acetate of soda or potash in absolute 

 alcohol ; but washing with neutral alcohol will generally 

 suffice. 



With certain objects this solution does not penetrate well. 

 This may be remedied by acidifying the solution, or, which 

 is better, by leaving the objects for some time before stain- 

 ing in acid alcohol. Anyway objects ought not to have an 

 alkaline reaction. If these precautions be taken, it will not 

 be necessary to use acid for washing out. 



The solution is not recommended as giving as good results 

 as hsemalum, and Mayer recommends it merely as a substi- 

 tute for Kleinenberg's, in cases in which an alcoholic h^matein 

 stain seems indicated, as being easy to prepare, and constant 

 in its eifects. 



262. Mayer's Haemastrontium [Grundziigc, Lee and Mayei;, 

 1910, p. 166). — 1 grm. hasmatein, 1 grm. aluminium chloride, 

 50 grm. strontium chloride, 600 c.c. alcohol of 70 per cent., 

 and (if desired) 0'25 g. citric acid. Prepare and use as 

 hsemacalcium. 



263. De GrKOOT's Aleoholie Haemalum (Zeit. wiss. Mile, xxix, 1912, 

 p. 182). — Mix 20 CO. of glycerin with 240 of alcohol of 70 per cent. 

 Take 4 c.c. of the mixture, 2 c.c. of hydrogen peroxide, and 05 grm. of 

 hsematoxylin, and dissolve with heat. Add 60 c.c. of the mixture, 4 grms. 

 of calcium chloride, and 2 grms. of sodium bromide. Dissolve, add 3 grms. 



