HiEMATEIN (hTEMA'I'OXYLIN) STAINS. 167 



268. Vanadium Hsematoxylin (Hbidenhain, JSncyclop. mile. 

 Teohnih., 1903, p. 518).— Add 60 c.c. of a 6 per cent, solution of lia3nia- 

 toxylin to a 0'26 per cent, solution of vanadate of ammonium (quantity 

 not stated ; should be 30 c.c, see Cohn in Anat. Hefie, xv, 189S, p. 302). 

 The mixture to be used after three or four days ; it will not keep over 

 eight days. To be used with sections of sublimate material. A strong 

 plasma stain for special purposes, especially mucus glands. 



270. Benda's Copper Hsematoxylin {Arch. milt. Anat., xxx, 1887, 

 p. 49). — See fourth edition. According to my experience, not to be 

 compared with iron hsematoxylin, and superfluous. 



271. Mallory's Phospho-molybdic Acid Hsematoxylin {Anat. 

 Anz., 1891, p. 375). — One part 10 per cent, phosplio-molybdic 

 acid solution, 1 part hgematoxylin, 100 parts water, and 6 to 

 10 parts chloral hydrate. Let the solution i-ipen for a week 

 in sunlight, and filter. Chiefly for central nervous system. 

 Sections should be stained for from ten minutes to one hour, 

 and washed out in two or three changes of 40 to 50 per cent, 

 alcohol. It is necessary that the solution should be saturated 

 with hEematoxylin in order to obtain the best results ; if a 

 good stain be not obtained at once, more hsematoxylin must 

 be added. Water must never be used for diluting it. 



See also Ribbbrt {Centralb. allg. Path., vii, 1896, p. 427 ; Zeit. wiss. 

 Mile, XV, 1898, p. 93), Patbllani (Mon. Zool. ItaL, xiii, 1902, p. 6), and 

 GoLOViN (Zeit. wiss. Mile, xix, 1902, p. 184). 



Saegbnt (Anat. Aiiz., xv, 1898, p. 214) quotes this stain, preceded by 

 mordanting for twenty-four hours in 5 per cent, sulphate of copper, as 

 Kbnyon's. 



KoDis {Arch. mih. Anat., lix, 1901, p. 211) takes hsematoxylin, 1 part; 

 molybdic anhydride, 1'5 ; water, 100 ; H^Os, 0'5, or a crystal of HgO. 



Police {Arch. Zool. Napoli, iv, 1909, p. 300) takes O'SS grm. hsema- 

 toxylin, 10 drops phospho-molybdic acid of 10 per cent., 10 grms. chloral 

 hydrate, and 100 grms. alcohol of 70 per cent. 



272. Malloey's Phospho-tungstic HBematoxylin (Journ. Exp. 

 Med., V, 1900, p. 19 ; Zeit. wiss. Mih., xviii, 1901, p. 178) : 



Hsematoxylin . . . . . 0*1 



Water 80-0 



10 per cent, solution of (Merck's) phospho- 

 tungstic acid ..... 20-0 

 Peroxide of hydi'ogen (U.S. Ph.) . . 0-2 



(Dissolve the hasmatoxylin, add the acid, then the peroxide.) 

 Stain sections two to twenty-four hours, wash out with water. 



