190 OHAPI'Eli XV. 



See also Flemming in Arcli. Aiiat. Phijs. Aiuot. AUth., lyiJ7, p. 175. 

 Never popular, this clumsy and uncertain prouess is now little used. 



301. Reinke's Orange Method {Arch. mile. Aiiat., xliv, 2, lo'Jl,p. 2(j2). 

 — To a concentrated aiiueous solution of gentian violet are added " a few- 

 drops " of a like solution of orange G. The solution precipitates in part , 

 owing to the formation of an imperfectly soluble " neutral " colour, but 

 becomes almost clear again if an excess of water be added. The 

 solution is not to be filtered, but the sections are to be stained in the 

 mixture made almost clear by addition of water. It is said that the 

 " neutral " solution may be preserved for f atare use by adding to it one 

 third of alcohol. After staining (sections previously stained with 

 safranin), yoii diiferentiate rapidly with alcohol and clear with clove oil. 



I have tried this process and obtained exactly the same results as with 

 Flemming's process, and so have other workers. 



Aenold's Orange Method {Arch. Zellforsch., iii, 1909, p. 434). — 

 Sections (of chrome material) are treated for five minutes with solution of 

 equal parts of iodine and iodide of potassium in alcohol of 40 per cent., 

 then washed and stained for 4 hours in saturated solution of safranin in 

 alcohol of 75 per cent. -. then washed and put for 5 to 15 minutes into 

 solution of seven parts of methylen blue, 0'5 of carbonate of soda and 100 

 of water, washed, dehydrated, and treated until pale blue with solution of 

 orange G. in oil of cloves. Cytoplasmic reticulum blue on orange ground, 

 nucleoli and centrosomes red. Instead of the safranin, basic fuchsin 

 may be taken. 



302. Bonney's Triple Stain [Virclioio's Arch., cxciii^ 1908, 

 p. 547, and elsewhere). — Stain sections (of acetic alcoliol or 

 sublimate material, not clirome or formol material) for two 

 minutes in a solution of 0'25 parts methyl violet and 1 part 

 pyronin in 100 of water. Wipe slide dry, and flood twice 

 with the following : 2 per cent, aqueous solution of orange &, 

 boiled and filtered^ is added drop by drop to 100 c.c. of 

 acetone, with agitation, until there is formed a flocculent 

 precipitate, which redissolves on farther addition of the 

 orange. Wash rapidly in pure acetone, and jjass through 

 xylol into balsam. Chromatin violet, cytoplasm red, con- 

 nective tissue yellow, keratin violet. Not adapted for blood 

 films. 



303. Bordeaux R. — An " acid " dye, giving a general stain 

 taking effect botli on chromatin and cytoplasm, and, I con- 

 sider, a very good plasma stain. I use for chrom-osmium 

 material a 1 per cent, solution, and stain for twelve to 

 twenty-four hours. The stain is sufficiently fast. 



