PLASMA STAINS WITH COAL-TAE DYES. 193 



Eosin is a specific stain for red blood-corpusoles, and also 

 for certain granules of leucocytes (see under " Blood "). 



The yolk of some ova takes the stain strongly, so that it 

 is useful in some embryological researches. 



311. Ehrlich's Indulin-Aurantia-Eosin, or Acidophilous 

 Mixture, or Mixture C, or Mixture for Eosinophilous Cells (from 

 the formula kindly sent me by Dr. G-eijblee). — Indulin, 

 aurantia, and eosin, of each two parts ; glycerin, thirty 

 parts. This gives a very thick, syrupy solution. To use it, 

 cover-glass preparations may be floated on to it ; or sections 

 on slides may have a fewdrops poured on to them, the slide 

 being laid flat till the stain has taken eifect (twenty-four hours 

 for Flemming material) . I flnd that with Plemming material 

 it gives a powerful and good stain, which is much more 

 resistant to alcohol than that of the Bhklich-Biondi mixture, 

 and is, therefore, much more adapted to ordinary work. 

 The stain keeps well. 



Israel (PraUih. Path. Hist., Berlin, 1893, p. 68) gives a more 

 complicated receipt. 



312. Methyl Green and Eosin (Calbeela, Morph. Jahrh., iii, 1877, 

 Heft 3, p. 625 ; List, Zeit. wiss. Mih., ii, 1885, p. 147 ; Balbiani, Ann. 

 Microgr., Paris, vii, 1895, p. 245 ; Rhumblee, Zeit. wiss. Zool., Ixi, 1895, 

 p. 38). — See early editions, 



313. Methylen Blue and Eosin (Chenzinsky, quoted from Zeit. 

 wiss. Mih., xi, 2, 1894, p. 269). 



Methylen bhie, sol. sat. in water . . . .40 

 Eosin, 0'5 per cent, in 70 per cent, alcohol . . 20 

 Distilled water, or glycerin . . .40 



This solution will only keep for about eight days. 

 Pianesb {ibid., xi, 1894, p. 345) adds a considerable proportion of 

 carbonate of lithia. 



See also the mixture of Beembe {Arch. mih. Anat., xlv, 1895, p. 446). 

 I have tried Chbnzinsky's mixture as a tissue stain, without good 

 results ; but see Rosin, Berliner hlin. Wochenschr., 1898, p. 251 ; Zeit. 

 wiss. Mih., xvi, 1899, p. 223, and xvii, 1900, p. 333. 



See also Laueent {Centralb. allg. Path., xi, 1900, p. 86 ; Zeit. wiss. Mik., 

 xvii, 1900, p. 201). 



314. Malloey's Eosin and Methylen Blue {Journ. med. Research, 

 January, 1904). — Sections of Zbnkbe material (other sublimate material 

 not so good) are stained for half to three quarters of an hour at 56° 0. 

 in 5 per cent, aqueous solution of eosin, rinsed and flooded with solution 



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