METHYfiEN BLUE. '2.11 



cannot be equally well obtained with toluidin blue or tliionin. 

 For staining pieces of tissue he takes : 



Toluidin blue, O'l per cent. sol. in phy- 

 siological salt solution . . .2 parts 

 Ammonium chloride 0'25 per cent, in 



water ...... 1 part 



Egg albumen . . . . . 1 ,, 



For injections he uses 1 part of the dye to 1000 of physio- 

 logical salt solution. 



Any of the methylen blue fixing methods may be employed 

 and the whole technique is the same. 



L. Martinotti {Zeil. iviss. MiJc, xxvii, 1910, p. 24) 

 recommends a polychrome toluidin blue, made by adding 0'5 

 per cent, of lithium carbonate to a 1 per cent, solution of the 

 dye and keeping till a purple-red tone appears. Or, a stock 

 solution made of 1 grm. toluidin blue, O'o grm. lithium 

 carbonate, glycerin 20 grms., alcohol 5 grms., and water 75. 



