'^■jO chapter XVIII. 



385. Merkel's Carmine and Indigo-Carmine in One Stain 



(Meekbl, Unters. anat. Anst. Bosioch, 1874; Month. Mic. Jourii., 1877, 

 pp. 212 and 317). 



Also NoREis and Shakbspbaee, Amei-.Joum. Med. Sci., January, 1877 ; 

 Merkel, Man. Mic. Journ., 1877, p. 242 ; Maesh, Section Cuttivy, p. 85 ; 

 'Ba.y'eb.'L, Arch. Mih. ^ual, xxiii, 18? 5, pp. 36, 37; Macalltjm, Trans. 

 Canad. Instii., ii, 1892, p. 222 ; Joiu-n. Boy. Mic. /S'oc, v, 1892, p. 698. 



386. Mayer's Carmalum (or Haemalum) and Indigo-Carmine 



in one Stain.— May ke [Mitth. Zool. Stat. Ncirjiel, xii, 1896, 

 p. 320) obtains very good results by taking a soltition of O'l 

 grm. of indigo-carmine in 50 c.c. of distilled water, or 

 .5 per cent, alum solution, and combining it with from four 

 to twenty volumes of carmalum or heemalum. 



387. Carmine and Picro-Indigo-Carmine (Ramon y Cajal, i?er. 

 (hi Gienc. vied., 18.95 ; Calleja, B.ev. trim. Microgr., ii, 1897, 

 p. 101 ; Zeit. tuiss. Milt., xv, 1899, p. 323). — For use after a 

 carmine stain, Ramon takes a solution of 0'25 grm. of 

 indigo-carmine in 100 grms. saturated aqueous solution 

 of picric acid. Stain {t<ectioiis) for five to ten minutes, 

 wash in weak acetic acid, then in water, then remove the 

 excess of picric acid with absolute alcohol, clear and 

 mount. 



Ramon also [Elemenlo.-i de Histulogia, 1897 ; quoted from 

 La Cellule, xix, 1901, p. 212) employs the picro-indigo 

 mixture after Magenta ; stain strongly in saturated solution 

 of magenta, rinse in water until no more colour comes away, 

 and pass into the indigo mixture. See also Boeuel, Anv. 

 Inst. Pasteur, 1901, p. 57, or Lee et Henneguy', Traite, 

 p. 268. 



388. Carmine and Anilin Blue (or Bleu Li;miere, or Bleu de 



Lyon) (DuvAr,, Precis de TecJiniqiie Microscujiique, 1878, p. 

 225). — Stain with carmine; dehydrate, and stain for a few 

 minutes (ten minutes for a section of nerve-centres) in a 

 solution of ten drops of saturated solution of anilin blue in 

 alcohol to 10 grms. of absolute alcohol. Clear with 

 turpentine, without further treatment with alcohol, and 

 mount in balsam. 



Other authors recommend, instead of anilin blue, bleu de 

 Lyon, dissolved in 70 per cent, alcohol acidulated with acetic 

 acid (Maukicio and Schulgin), or bleu lumiere. 



