232 CHAPTER xviit. 



{Verh. Berl. Phys. Gses., 1887); Gieeke {Zeit. win.s. Mik., i, 

 1884, p. 505). 



Sections should be very well washed before being passed 

 from eosin into hsematoxylin or the reverse, as eosin very 

 easily precipitates liEematoxylin. 



For the complicated and superfluous mixtures of Renadt 

 and of EvEEAED, Demode and Massaet, see Foi/s Lehrbuch, 

 p. 196; Ann. Inst. Pasteur, vii, 1893, p. 166, or early 

 editions. 



394. Hsematoxylin and Congo. — See § 305. 



395. Hesmatoxyliii and Safrauin. — Rabl (Morph. Jahrb., x, ISSi, 

 p. 215) stained very liyhtlij with very dilute Dblafield's hiematoxylin for 

 twenty-four hours, then for some hours in (PriTZNBB's) safranin and 

 washed out with pure alcohol. The plasma stain is here given by the 

 hsematoxylin. 



Similarly Regaud, Verh. Anat. Ges., xiv, 1900, p. ] 12. 



FoA [Festschr. VircJiow, 1891, p. 481) stains in a mixture 

 of 25 c.c. of Bohmer's hfematoxylin, 20 of 1 per cent, solution 

 of safranin, and 100 of water for one to three minutes. 



396. Hsematoxylin and Saurefuchsin. — Stain first with iron 

 hasmatoxylin or hsemalum, then stain (sections) in 0'5 per 

 cent, aqueous solution of Saurefuchsin, dehydrate and 

 mount. 



397. Hsematoxylin and Saurefuchsin and Orange. — Proceed 



as above, using for the second stain the following mixture : 

 Saurefuchsin, 1 grm. : orange, 6 grms. ; rectified spirit, 

 60 CO. ; water, 240 c.c. (from Squiee's Methods and Formulie, 

 p. 42) . Using orange Gr. (not mentioned by Squiee), I have 

 had very good results. 



The method of Cavazzani {Biforma Med., Napoli, 1893, 

 p. 604 ; Zeit. iviss. Mik., xi, 3, 1894, p. 344) is far too com- 

 plicated. 



398. Hsematoxylin and Picro-Saurefuchsin (van Gieson, New 

 York Med. Journ., 1889, p. 57 ; quoted from Moeller, Zeit. 

 wiss. Mik., XV, 2, 1898, p. 172, which see for fm-ther details). 

 Proceed as above, using for the second stain the picro- 



