316 CHAPTER XXV. 



KosTANECKi and Siedlecki {ibid., xlviii^ 1896, p. 184) 

 employed concentrated sublimate solution, or 8 per cent, 

 nitric acid or mixtures of these two, for ovarian ova. 



Van Beneden and Neyt (Bull. Acad. Belg., 1887, p. 214) 

 took equal parts of alcohol and acetic acid. Bovkui {.Jena 

 Zeit., xxi, 1887, p. 423) fixed in his picro-acetic acid, § 95 — 

 a clearly inadequate method. Gulick {Arch. Zellforsch., vi, 

 1911) has " fixed " ova of HeteraJiit for 22 hours in one third 

 saturated picric acid with -3 per cent, of glacial acetic acid, 

 and had them develop in alcohol of 70 per cent, to stages 

 representing a normal development of several weeks. 



Boeing {Arch. Zellforsch., iv, 1909, p. 121) spreads ova of 

 AscarU on a layer of Mayer's albumen on a slide, sets the 

 albumen with a drop of formol, fixes with 4 parts of alcohol 

 to 1 of acetic acid, stains in alcoholic hydrochloric acid 

 carmine, and mounts in glycerin. 



Aetom {Zeit. iviss. Mile., xxv, 1908, p. 5) freezes segments 

 of the uteri of Ascaris iu salt water, and cuts them with the 

 freezing microtome into di.sks 30 fi thick, and fixes these with 

 divers liquids. 



Ceefontaine {ibid., xxix, 1912, p. 305) brings fixed ova 

 from alcohol into absolute alcohol with 1 per cent, of clove 

 oil, evaporates this down to one tenth, puts into absolute 

 alcohol with 5 per cent, of clove oil, evaporates again down 

 to one tenth, then into the same with 5 per cent, of collodion 

 added, evaporates almost entirely away, and passes through 

 cedar oil into paraffin. 



Echinodermata, Coelenterata, and Porifera. 

 See the chapter on " Zoological Methods." 



