328 ci-iAPi'ioR XXVI. 



Bihliogr. Anut., Nov. Gib, 1897, p. 278, uud Juimi. (h; I'Annt., 

 xxxvi, 1900, p. 22, and under " Mitochondria/' next §. 



Altmann {Stadien iiber die Zdle, 1886; Die Elemeiiturorgciuismen 

 Leipzig, 1890 ; Arch. f. Auat. u. Entwiclcel, 1892, p. 22.3; also Zeit. f. 

 wiss. Mile, vii, 2, 1890, p. 199; ix, 3, 1893, p. 331; and L. and R. ZOJA, 

 in Mem. B. Isi. Lombardo di Sci. e Letteiv , xvi, 3, vii, p. 237) demon- 

 strates his " Bioblasts " by fixing for twenty -four liours in a, mixture of 

 equal parts of 5 per cent, bichromate of potash and 2 per cent, osmic 

 acid, imbedding in paraiBn, staining sections for a minute on the slide 

 held over a flame with a solution of 20 grms. of acid fuchsin in 100 c.c. 

 of anilin water (§ 280), and washing out with saturated alcoholic solution 

 of picric acid diluted with 2 volumes of water, heat being used as before 

 to aid the differentiation, and finally clearing with xylol and mounting 

 in balsam. See hereon the critique of Fischer, in his Fixii-ung, 

 Faerbiing, u. Bau des Protoplusmas, pp. 108, 29.5 (these granules 

 mainly artefacts). 



653. Micocliondria (Chondriosomes, Chondriokonts, Chromidia, 

 Ergastoplasni, etc.).^Tliese formations are fixed, more or le.ss 

 abundantly, by most of the usual fixatives. But some 

 kindfe of them seem to be attacked by organic acids ; so 

 that it is well to reduce the proportion of these in mixtures. 

 Thus Benda for this purpose makes up Flemming*s strong- 

 mixture with only three to six drops of acetic acid to 15 c.c. 

 of the chromic, and 4 c.c. of the osmic. Meves [Encycl. 

 inik.. Techn., 1910, i, p. 476) takes 15 c.c. of chromic acid of 

 0'5 to 1 per cent., containing 1 per cent, of sodium chloride, 

 with 3 to 4 c.c. of osmic acid of 2 per cent., and three to four 

 drops of acetic acid. Similarly, Duesberg {Arch. Zellforsch., 

 \v, 1910, p. 605). Champy [Arch. d'Anat. Mic, xiii, 1911, 

 p. 55) takes 7 parts of bichromate of potash of 3 per cent., 

 7 of chromic acid of 1 per cent., and 4 of osmic acid of 2 

 per cent. Or iodide of sodium 15 grms. ; water 800 grms. ; 

 formol 200 grms. ; iodide of mercury to saturation. Or 

 simply formol. Some workers take Altmann's osmic acid and 

 bichromate, §43; so Meves, Arch. mili. Anat., Ixxvi, 1911, 

 p. 683. 



Eegaud {Arch. AnaL mic, xi, 1910, fasc. 2 and 3) fixes 

 either in 100 parts of 3 per cent, bichromate of potash with 

 20 of formol and 5 of acetic acid; or 80 parts of the bi- 

 chromate to 20 of formol zvilhout acetic acid; or in 20 parts 

 saturated aqueous picric acid with 5 of formol ; or in formol 

 of 10 per cent. ; and in either case mordants for one to 



