330 CHAPTEB XXVI. 



See also some modifications by Kolsteb, Buitr. yath. Auat., li, 1911, 

 p. 209, consisting in fixation and mordanting in certain chrome alum 

 and chromium fluoride mixtures. 



DuESBEBG (loc. cit. ante) lias found that the treatment with the 

 chromic and pyrohgneous acid and bichromate may be suppressed — 

 with advantage. 



SziJTS employs the aluminium-alizai-in stain given § 335, in lieu of 

 the iron-alizarin for Benda's process. 



Some workers (so Meves) prefer to harden as Benda, but 

 to stain with iron haematoxylln instead of by the alizarin 

 process ; the special hardening rendering the hsematoxylin 

 stain sufficiently specific. Thus also Dingler, Arch. Zell- 

 forsch., iv, 1910, p. 673. 



Aenold [ibid., viii, 1912, p. 256) stains first with iron 

 hematoxylin, differentiates, stains for twenty to thirty min- 

 utes with saturated aqueous solution of thionin, passes up 

 to absolute alcohol, stains for two minutes with Orange G. 

 dissolved in clove oil, and passes through xylol into balsam. 

 Chromatin blue, chondriosomes black. 



Pbnsa {ihid. p. 612) has studied the mitochondria and chloroplasts in 

 plant cells by Ramon y Oajal's silver metlicd for neurofibrils, applied 

 to sections of fresh tissues (a few minutes to an hour in silver of 1 to 

 2 per cent., reduction for ten minutes to an hour in a hydroquinon 

 bath). 



Renaut (Goniptes rend.,c\ii, 1911, p. 636) demonstrates mitochondria 

 in fresh cartilage cells by mounting sections in a mixture of artificial 

 serum and saturated aqueous solution of methyl violet 5 B. 



