TEGIJMENTARY OHGANS. 333 



(3) Hsemalum for two hours, neutral orcein as above for 

 ten to twenty minutes. 



More recently Unna advocates the process mentioned 

 last §. 



See alse Ranviee, Arch. Anat. Mihr., iii, 1899, p. 1. 



657. Keratohyalin. — The keratohyalin granules of the cells 

 of the stratum granuluaum are soluble in mineral acids, and 

 can. be digested in pepsin. They can be stained with picro- 

 carmine, alum hematoxylin, van Glieson's mixture, or Unna's 

 Wasserblau-orcein, last §. Fick [Centralh. allg. Path., xiii, 

 1902, p. 987 ; Zeit. iviss. Milt., xx, 1 903, p. 222) stains sections 

 of alcohol material for three to four minutes in concentrated 

 aqueous solution of Kresylechtviolett, differentiates in alcohol, 

 clears in xylol, and mounts in balsam. 



See also Unna, Monatsschr. pralct. Vevm., xx, 1895, p. 69 ; the article 

 " Haut " in the Encycl. mil;. Technik. ; and Unna and Golodbtz, 

 Monatsschr. pralct. Devtn., xlix, 1909, p. 95 ; Laffont, Bihl. Anat., 1909, 

 p. 209. 



For Tn'chohyaUn, see Gavazzeni, Monatsschr. praM. J)erm.,xlvii, 1908, 

 p. 229. 



658. Eleidin. — To demonstrate the stratum gramilosum and 

 the eleidin granules Ranvike [Arch. Anat. Micr., iii, 1899, 

 p. 1) hardens with alcohol, stains with picro-carmine, and 

 treats with lime-water. The cells swell and show up the 

 granules, which do not change. See loe. cit., other methods 

 for the study of skin. 



Buzzi (see Encycl. mile. Technik., article " Haut ") stains 

 sections for a few minutes in a watchglassful of water with 

 2 to 3 drops of 1 per cent. Congo red. Similarly Weiden- 

 EiacH, Arch. mih. Anat., Ivii, 1901, p. 583. Other authors 

 recommend nigrosin, or Wasserblau, or orcein. 



See also Joseph, " Dermatohist. Technik," Berlin, 1 905, 

 and Deeuw, Med. Klinilc, Berlin, 1907, Nos. 27 and 28. 



For Chnlesterin see Golodetz and Unna, Monatsschr. prakt. 

 Derm., xlvii, 1908, p. 1. 



659. Horn, Hair, and Nails — The elements of hairs and nails 

 may be isolated by prolonged maceration in 40 per cent, 

 potash solution, or by heating with concentrated sulphuric 

 acid. See also von Nathusius, Zool. Anz., xv, 1892, p. 395. 



