334 CHAPTER XXVII. 



Horny tissues stain well in safranin or gentian violet 

 (Reinkb, Avch.f. mih. Anat., xxx, 1887, p. 183; Ernst, iltid., 

 xlvii, 1896, p. 669; Rabl, ihid., xlviii, 1896, p. 489). 



Unna [op. cit. last §, p. 598) stains the tyrosin-bearing 

 keratin in sections of skin for a few seconds or minutes in 

 a mixture of 5 parts of Millon's reagent, 5 of water, 

 and 1 of glycerin, treats shortly with nitric acid of 25 per 

 cent., and mounts in balsam. 



660. Skin-nerves and Nerve-endings. — Impregnate with gold 

 chloride. See Chap. XVIT, especially § 365. 



661. Tactile Corpuscles. — See §§ 364-366.— Gold methods 

 are indicated. See also Ranvier, Traite, p. 919 ; Langee- 

 HANS, Arch. mik. Anat., 1873, p. 730 ; KuLTSCHizKy, ibid., 

 1881, p. 358; and Smienow, Intern. Monats.schr. f. Anat., 

 etc., X, 1893, p. 241, who recommends, besides the gold 

 method of Lowit, the rapid bichromate of silver method of 

 Golgi. 



662. Corpuscles of Herbst and Corpuscles ofGrandry. — Dogiel 

 (Arch. Anat. u. Entwichel., 1891, p. 182) has used the methylen 

 blue method. Four per cent, solution of methylen blue, 

 warmed to 40° 0., is injected into blood-vessels of the heads 

 of ducks or geese ; pieces of skin are removed from the 

 beaks, sectioned in pith, and the sections brought on to slides 

 and moistened with aqueous or vitreous humour from the 

 animal and left for ten to thirty minutes exposed to the air, 

 then brought into picrate of ammonia, and treated as 

 described § 343. Gebbkg [Intern. Monatsschr. Anat., x, 

 1898, p. 205) made use of a method of Aenstein, according 

 to which pieces of skin are put for twenty-four hours into 

 lime-water, the horny layer removed, the pieces treated for 

 five minutes with 0'25 per cent, gold chloride, reduced in 

 water, and the precipitate that forms on them removed 

 by putting into 0"25 per cent, cyanide of potassium and 

 brushing. 



NowiK [Anat. An?.., xxxvi, 1910, p. 217) takes Unna's 

 Orcein-wasserblau mixture (Wasserblau O.D., 1 part, orcein 

 1, acetic acid 5, glycerin 20, alcohol 50, water 100) and 

 adds to it 1 part more of orcein. To 10 c.c. of this he adds 



