338 



CHAfTElt XXVIK. 



salt solatioiij and exaininud pieces of njuscle in SGi-iiin of the 

 animal, afterwards fixing with picrate of ammonia and 

 mounting in glycerin jelly. 



The procedure of Dogiel has been given, § 342. 



669. Nerve-endings— the Gold Method. — Fischei; {Airh. mik. 

 Auat., 1876, p. 36-5) used the method of Lowit. 



BiEDEBMANN (last section) recommends for Antaeus a 

 similar procedure, the preliminary treatment with formic acid 

 being omitted, and the muscles being put for a couple of days 

 into glycerin after reduction in the acid. 



Eanviee {Traite, p. 813) finds that for the study of the 

 motor terminations of Vertebrates the best method is his 

 lemon-juice process (§ 366). 



See also the methods of Apathy, §§ 868, 371. 



670. Nerve-endings — the Silver Method. — Ran vie u employs 

 it as follows {ibid., p. 810) : Portions, of muscle (gastro- 

 cnemius of frog) having been very carefully teased out in 

 fresh serum, are treated for ten or twenty seconds with nitrate 

 of silver solution of 2 to 3 per 1000, and exposed to bright 

 light (direct sunlight is best) in distilled water. As soon 

 as they have become black or brown they are brought into 1 

 per cent, acetic acid, where they remain until they have 

 swelled up to their normal dimensions. They are then ex- 

 amined in a mixture of equal parts of glycerin and water. 



This process gives iiegraiwe images, the muscular substance 

 being stained brown, and the nervous arborescence unstained. 

 The gold process gives positive images, the nervous structures 

 being stained dark violet. 



671. Nerve-endings — the Bichi-omate of Silver Method. — The 



rajjid method of Golqi has been used by Eamon y Cajal 

 for the terminations of nerves and tracheaB in the muscles 

 of insects. See Zeit. iviss. Mil-., vii, 1890, p. 332, or fourth 

 edition. A modification is used by Wondeeee, Arch. mik. 

 Anat., Ixxi, 1908, p. 523. 



672. Muscle-spindles. — See Cilimbauis, Arch. mik. Anat., 

 Ixxv, 1910, p. 692. Principally intra vitavi methylen blue, 



