340 OHAPTEH XXVIII. 



fixed with osmio acid of 2 per cent, and impregnates it with 

 gold by the method of Nabias, and mounts in glycerin. 



Eaja. — IwANZOFF [Bnll. 8oc. Nat. Moscou, ix, 1895, p. 74) 

 fixes the organ in the tail of Raja with liquid of Flemmiiig, 

 stains with h^macalcium and eosin, and makes paraffin 

 sections. 



Ballowitz {Anat. Hefte, 1 Abth., vii, 1897, p. 285) finds 

 the method of Golgi excellent for this organ. He also makes 

 sections after fi.xing in saturated solution of sublimate (in 

 sea-water), or in liquid of Flemming, and examines them in 

 water. Methylen-blue may be used, intra vitam. Gold is 

 little good. 



Gymnotus. — Ballowitz {Encycl. mile. Technilc, p. 303) fixes 

 with Flemming, and makes sections. He also commends 

 impregnation with gold chloride, but not the Golgi method. 



Malapterurus. — Ballowitz {ibid., p. 202) fixes with picro- 

 sublimate, with Flemming, or with various mixtures of 

 bichromate, sublimate, and formol, and uses gold chloride 

 and Golgi impregnations. He macerates in liquid of Miiller 

 or saturated aqueous solution of picric acid. 



Tendon. 



674. Tendons.— ItETTBEEK [G. R. 8oc. Biol, x, 1898, p. 580) 

 fixes in equal jjarts of saturated solutions of sublimate and 

 picric acid, puts for 1 to 3 days into saturated picric acid 

 with 2 to 3 per cent, of sodium chloride, to remove the 

 mucin, and imbeds in paraffin. 



674a. Union of Muscle and Tendon. — For this see Eettkkbk 

 and Lelievke, C. R. Soc. Biol., 1911, No. 12 (orcein for 

 24 hours, followed by iron hasmatoxylin) ; ;ind Schultze 

 {Vcrlt. pJiys. med. Ges. Wiirzhurg, 1911, p. 33) (treats for a 

 day or two with a mixture of equal parts of 2 per cent, 

 bichromate of potash and alcohol, in the dark, then for 

 2 days with 0'5 per cent, solution of hajmatoxylin in alcohol 

 of 70 per cent., then with Van Gieson's picro-saiirefuchsin). 



675. Corpuscles of Golgi (Ranviee, Traitc, p. 929). — Take 

 the tendon of the anterior and superior insertion of the 

 gemini muscles of the rabbit. Treat it by the formic acid 



