MUSCLE AND TENDON (nEEVE-RNDTNGS). 341 



and gold method (§ 365), and after reduction scrape with a 

 scalpel, in order to remove the muscle-fibres that mask the 

 musculo-tendinous organs. 



Maechi's methods for the tendons of the motores bulbi 

 oculi [Archivio per le Bcienze Mediche, vol. v, No. 15). — The 

 enucleated eyes, together with their muscles, were put for 

 not less than three days into 2 per cent, bichromate of 

 potash. The muscles and tendons were then carefully 

 dissected out, stained with gold chloride and osmic acid 

 (G-OLGi's method), and by the method of Manpeedi, § 368. 

 Mount in glycerin. The methods only succeed completely 

 during fine, sunny weather. 



RuFFiNi {Atti B. Ace. Lincei Roma Bend. [5], i, 1892, 

 p. 442) recommends the method of Fischer. 



CiACCio {Mem. B. Ace. 8ci. Bologna [4], t. x, 1890, p. 301) 

 puts tendons of Amphibia into O'l per cent, hydrochloric 

 acid or 0'2 per cent, acetic acid until transparent ; then for 

 five minutes into a mixture of 0"1 per cent, gold chloride 

 and 0"1 per cent, potassium chloride ; then back into the 

 acetic acid, for a day in the dark, and for two or three 

 hours more in the sunlight. When they have become 

 somewhat violet they are put for a day into O'l per cent, 

 osmic acid, and finally mounted in glycerin acidulated with 

 0"5 per cent, of acetic or formic acid. 



DoGiEL (Arch. mile. Anat., Ixvii, 1906, p 638) stretches 

 tendons of eye-muscles on cardboard with hedgehog spines, 

 puts for 4 or 5 days into nitrate of silver of 1 to 2 per cent., 

 reduces for a day in pyrogallic acid with foi'mol, and imbeds 

 in celloidin. 



Smooth Muscle. 



676. Tests for Smooth Muscle. — Picro-siiurefuchsin, § 299, 

 stains muscle yellow, connective tissue red. 



Picro-nigrosin, § 325, stains muscle yellowish, connective 

 tissue blue. 



Unna {Encycl. inih. Technik., article " Kollagen ") stains 

 for twenty-four hours in orcein 1 part, Wasserblau 0'25, 

 alcohol 60, glycerin 10, water 30, which gives muscle in a 

 mixed tone, collagen blue, elastin reddish. See also a com- 

 plicated process with methylen blue in Monatssch. praJd. 



