342 CHAPTER XXVIII. 



Dermatol., x\x, 1894, p. 533, and another witli orcein, 

 hmmatein, saurefuchsin and picric acid. 



Retteeee {G. R. Soc. Biol, 1887, p. 645) fixes in 10 vols, 

 of alcoliol with one of formic acid, washes well and stains in 

 alum carmine. Muscle red, connective tissue unstained. 



677. General Structure. — Werner {Hifit. d. glatten Mnsru- 

 Intvr, Dorpat, 1894, p. 22) fixes stretched intestine or bladder 

 in riemmina:, washes well and stains in Heidenhain's chrome 

 liasmatoxylin, § 265. For demonstrating intercellular spaces, 

 fresh intestine is put for 24 hours into oil, at 37° C, then for 

 12 hours into Flemming, and for 4 to 6 into chromo- acetic 

 acid. 



678. Isolation of Fibres. — Gage's methods, see §§ 527, 544, 

 and 536. 



MoBius, muscle of Cardium, see § 535. 



Ballowitz, muscle of Cephalopoda, see Arch. mile. Anat., 

 xxxix, 1892, p. 291. 



ScHULTZ (Arch. Anaf. Phyf., Phys. Ahth., 189.5-6, p. 521) 

 puts muscle of Vertebrates for twenty-four hours into 10 

 per c^nt. nitric acid, rinses with water, and brings pieces 

 for six to eight days (in the dark at first) into a mixture of 

 equal parts of g— per cent, osmic acid and ^ per cent, acetic 

 acid, teases and mounts in glycerin. 



For smooth muscle of Vermes, see ArATiiT, Zeit f. in',^.^. 

 Mil-., X, 1893, pp. 36, 319, and § 538, ante. 



679. Iris.— DoGiEL [Arch. mil-. Anat., 1886, p. 403) puts 

 the anterior half of an enucleated eye for some days into a 

 mixture of two parts one-third alcohol and one part 0"5 per 

 cent, acetic acid. The iris can then be isolated, and split 

 from the edge into an interior and posterior plate, and these 

 stained according to the usual methods. 



See also Koganei, Arch. mil-. Anat., 1885, p. 1 ; Canfield, 

 ibid., 1886, p. 121 ; and Dostoiewsky, ihid., p. 91. 



680. Bladder of Frog, Innervation of (Wolff, Arch. f. mile. 

 Anat., 1881, p. 362). — A frog is killed and a solution of gold 

 chloride of 1 : 20,000 injected into the bladder through the 

 anus. (If the injection flows out on removal of the syringe, 



